Two-photon bioimaging utilizing supercontinuum light generated by a high-peak-power picosecond semiconductor laser source

Hiroyuki Yokoyama, Hiroshi Tsubokawa, Hengchang Guo, Jun Ichi Shikata, Ki Ichi Sato, Keijiro Takashima, Kaori Kashiwagi, Naoaki Saito, Hirokazu Taniguchi, Hiromasa Ito

Research output: Contribution to journalArticlepeer-review

27 Citations (Scopus)

Abstract

We developed a novel scheme for two-photon fluorescence bioimaging. We generated supercontinuum (SC) light at wavelengths of 600 to 1200 nm with 774-nm light pulses from a compact turn-key semiconductor laser picosecond light pulse source that we developed. The supercontinuum light was sliced at around 1030- and 920-nm wavelengths and was amplified to kW-peak-power level using laboratory-made low-nonlinear-effects optical fiber amplifiers. We successfully demonstrated two-photon fluorescence bioimaging of mouse brain neurons containing green fluorescent protein (GFP).

Original languageEnglish
Article number054019
JournalJournal of Biomedical Optics
Volume12
Issue number5
DOIs
Publication statusPublished - 2007 Sep

Keywords

  • Frequency conversion
  • Multiphoton processes
  • Picosecond phenomena
  • Semiconductor lasers
  • Ultrafast nonlinear optics fluorescence microscopy

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Biomedical Engineering

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