TY - JOUR
T1 - Two pairs of mushroom body efferent neurons are required for appetitive long-term memory retrieval in drosophila
AU - Plaçais, Pierre Yves
AU - Trannoy, Séverine
AU - Friedrich, Anja B.
AU - Tanimoto, Hiromu
AU - Preat, Thomas
N1 - Funding Information:
We are grateful to Valérie Goguel and Aurélie Saint-Amaux for assistance in obtaining the qPCR data. We thank Ann-Shyn Chiang, Aaron DiAntonio, Barret Pfeiffer, and Gerry Rubin for providing useful fly strains or antibodies, and Yoshi Aso for valuable discussion. We thank the TRiP at Harvard Medical School (NIH/NIGMS R01-GM084947) for providing transgenic RNAi fly stocks. We thank Valérie Goguel and Gunnar Newquist for critical comments on the manuscript. S.T. was supported by a fellowship from the Fondation pour la Recherche Médicale. T.P. was supported by a grant from the French Agence Nationale de la Recherche. H.T. received financial support from Bernstein Focus Learning via BMBF and from the Max-Planck-Gesellschaft.
PY - 2013
Y1 - 2013
N2 - One of the challenges facing memory research is to combine network- and cellular-level descriptions of memory encoding. In this context, Drosophila offers the opportunity to decipher, down to single-cell resolution, memory-relevant circuits in connection with the mushroom bodies (MBs), prominent structures for olfactory learning and memory. Although the MB-afferent circuits involved in appetitive learning were recently described, the circuits underlying appetitive memory retrieval remain unknown. We identified two pairs of cholinergic neurons efferent from the MB α vertical lobes, named MB-V3, that are necessary for the retrieval of appetitive long-term memory (LTM). Furthermore, LTM retrieval was correlated to an enhanced response to the rewarded odor in these neurons. Strikingly, though, silencing the MB-V3 neurons did not affect short-term memory (STM) retrieval. This finding supports a scheme of parallel appetitive STM and LTM processing
AB - One of the challenges facing memory research is to combine network- and cellular-level descriptions of memory encoding. In this context, Drosophila offers the opportunity to decipher, down to single-cell resolution, memory-relevant circuits in connection with the mushroom bodies (MBs), prominent structures for olfactory learning and memory. Although the MB-afferent circuits involved in appetitive learning were recently described, the circuits underlying appetitive memory retrieval remain unknown. We identified two pairs of cholinergic neurons efferent from the MB α vertical lobes, named MB-V3, that are necessary for the retrieval of appetitive long-term memory (LTM). Furthermore, LTM retrieval was correlated to an enhanced response to the rewarded odor in these neurons. Strikingly, though, silencing the MB-V3 neurons did not affect short-term memory (STM) retrieval. This finding supports a scheme of parallel appetitive STM and LTM processing
UR - http://www.scopus.com/inward/record.url?scp=84887615404&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84887615404&partnerID=8YFLogxK
U2 - 10.1016/j.celrep.2013.09.032
DO - 10.1016/j.celrep.2013.09.032
M3 - Article
C2 - 24209748
AN - SCOPUS:84887615404
SN - 2211-1247
VL - 5
SP - 769
EP - 780
JO - Cell Reports
JF - Cell Reports
IS - 3
ER -