Two methods of temperature control for single-molecule measurements

Matthew A.B. Baker; Yuichi Inoue; Kuniaki Takeda; Akihiko Ishijima; Richard M. Berry

doi:10.1007/s00249-010-0667-y

Two methods of temperature control for single-molecule measurements

Matthew A.B. Baker, Yuichi Inoue, Kuniaki Takeda, Akihiko Ishijima, Richard M. Berry

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

Modern single-molecule biophysical experiments require high numerical aperture oil-immersion objectives in close contact with the sample. We introduce two methods of high numerical aperture temperature control which can be implemented on any microscope: objective temperature control using a ring-shaped Peltier device, and stage temperature control using a fluid flow cooling chip in close thermal contact with the sample. We demonstrate the efficacy of each system by showing the change in speed with temperature of two molecular motors, the bacterial flagellar motor and skeletal muscle myosin.

Original language	English
Pages (from-to)	651-660
Number of pages	10
Journal	European Biophysics Journal
Volume	40
Issue number	5
DOIs	https://doi.org/10.1007/s00249-010-0667-y
Publication status	Published - 2011 May

Keywords

Bacterial flagellar motor
Microscopy
Myosin
Single molecule
Temperature

ASJC Scopus subject areas

Biophysics

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title = "Two methods of temperature control for single-molecule measurements",

abstract = "Modern single-molecule biophysical experiments require high numerical aperture oil-immersion objectives in close contact with the sample. We introduce two methods of high numerical aperture temperature control which can be implemented on any microscope: objective temperature control using a ring-shaped Peltier device, and stage temperature control using a fluid flow cooling chip in close thermal contact with the sample. We demonstrate the efficacy of each system by showing the change in speed with temperature of two molecular motors, the bacterial flagellar motor and skeletal muscle myosin.",

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AU - Inoue, Yuichi

AU - Takeda, Kuniaki

AU - Ishijima, Akihiko

AU - Berry, Richard M.

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AB - Modern single-molecule biophysical experiments require high numerical aperture oil-immersion objectives in close contact with the sample. We introduce two methods of high numerical aperture temperature control which can be implemented on any microscope: objective temperature control using a ring-shaped Peltier device, and stage temperature control using a fluid flow cooling chip in close thermal contact with the sample. We demonstrate the efficacy of each system by showing the change in speed with temperature of two molecular motors, the bacterial flagellar motor and skeletal muscle myosin.

KW - Bacterial flagellar motor

KW - Microscopy

KW - Myosin

KW - Single molecule

KW - Temperature

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