Two methods of temperature control for single-molecule measurements

Matthew A.B. Baker, Yuichi Inoue, Kuniaki Takeda, Akihiko Ishijima, Richard M. Berry

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

Modern single-molecule biophysical experiments require high numerical aperture oil-immersion objectives in close contact with the sample. We introduce two methods of high numerical aperture temperature control which can be implemented on any microscope: objective temperature control using a ring-shaped Peltier device, and stage temperature control using a fluid flow cooling chip in close thermal contact with the sample. We demonstrate the efficacy of each system by showing the change in speed with temperature of two molecular motors, the bacterial flagellar motor and skeletal muscle myosin.

Original languageEnglish
Pages (from-to)651-660
Number of pages10
JournalEuropean Biophysics Journal
Volume40
Issue number5
DOIs
Publication statusPublished - 2011 May

Keywords

  • Bacterial flagellar motor
  • Microscopy
  • Myosin
  • Single molecule
  • Temperature

ASJC Scopus subject areas

  • Biophysics

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