Two isoforms of a human actin-related protein show nuclear localization and mutually selective expression between brain and other tissues

Masahiko Harata, Ryo Mochizuki, Shigeki Mizuno

Research output: Contribution to journalArticlepeer-review

38 Citations (Scopus)

Abstract

Actin-related proteins (Arps), which are divergent, but apparently homologues to actin, are categorized into 10 classes. While Arps belonging to classes 1-3 were found to be localized in the cytoplasm across eukaryotic phyla, other classes of Arps were found mostly in invertebrates and suggested to contribute to structural modulation of chromatin. Here we report the identification and the characterization of two human isoforms of an Arp not belonging to classes 1-3, which we designated hArpNα and hArpNβ. Both proteins were expressed in HeLa cells and they were found localized within the nucleus. Most interestingly, in different human tissues, hArpNα and β were found to be expressed mutually exclusively, and the expression of hArpNα was absolutely restricted to the brain. These findings suggest that, in vertebrates, members of distantly related Arps might have tissue-specific functions in the nucleus, possibly through structural modulation of chromatin.

Original languageEnglish
Pages (from-to)917-923
Number of pages7
JournalBioscience, Biotechnology and Biochemistry
Volume63
Issue number5
DOIs
Publication statusPublished - 1999 Jan 1

Keywords

  • Actin-related protein
  • Chromatin remodeling
  • Chromatin structure
  • Tissue-specific expression

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biotechnology
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

Fingerprint Dive into the research topics of 'Two isoforms of a human actin-related protein show nuclear localization and mutually selective expression between brain and other tissues'. Together they form a unique fingerprint.

Cite this