TY - JOUR
T1 - Two distinct soil disinfestations differently modify the bacterial communities in a tomato field
AU - Nakayasu, Masaru
AU - Ikeda, Kyoko
AU - Yamazaki, Shinichi
AU - Aoki, Yuichi
AU - Yazaki, Kazufumi
AU - Washida, Haruhiko
AU - Sugiyama, Akifumi
N1 - Funding Information:
This research was funded in part by grants from the Core Research for Evolutional Science and Technology, Japan Science and Technology Agency (JPMJCR17O2 to Y.A. and A.S.), the Research Institute for Sustainable Humanosphere (Mission 1), and the Research Unit for Development of Global Sustainability, Kyoto University. H.W. and K.I. were partly supported by Commercialization Promotion Course II of Kigyonomori from KYOTO Industrial Support Organization 21.
Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2021/7
Y1 - 2021/7
N2 - Reductive soil disinfestation (RSD) and soil solarization (SS) were evaluated based on environmental factors, microbiome, and suppression of Fusarium oxysporum in a tomato field soil. Soil environmental factors (moisture content, electric conductivity, pH, and redox potential (RP)) were measured during soil disinfestations. All factors were more strongly influenced by RSD than SS. 16S rRNA amplicon sequencing of RSD-and SS-treated soils was performed. The bacterial communities were taxonomically and functionally distinct depending on treatment methods and periods and significantly correlated with pH and RP. Fifty-four pathways predicted by PICRUSt2 (third level in MetaCyc hierarchy) were significantly different between RSD and SS. Quantitative polymerase chain reaction demonstrated that both treatments equally suppressed F. oxysporum. The growth and yield of tomato cultivated after treatments were similar between RSD and SS. RSD and SS shaped different soil bacterial communities, although the effects on pathogen suppression and tomato plant growth were comparable between treatments. The existence of pathogen-suppressive microbes, other than Clostridia previously reported to have an effect, was suggested. Comparison between RSD and SS provides new aspects of unknown disinfestation patterns and the usefulness of SS as an alternative to RSD.
AB - Reductive soil disinfestation (RSD) and soil solarization (SS) were evaluated based on environmental factors, microbiome, and suppression of Fusarium oxysporum in a tomato field soil. Soil environmental factors (moisture content, electric conductivity, pH, and redox potential (RP)) were measured during soil disinfestations. All factors were more strongly influenced by RSD than SS. 16S rRNA amplicon sequencing of RSD-and SS-treated soils was performed. The bacterial communities were taxonomically and functionally distinct depending on treatment methods and periods and significantly correlated with pH and RP. Fifty-four pathways predicted by PICRUSt2 (third level in MetaCyc hierarchy) were significantly different between RSD and SS. Quantitative polymerase chain reaction demonstrated that both treatments equally suppressed F. oxysporum. The growth and yield of tomato cultivated after treatments were similar between RSD and SS. RSD and SS shaped different soil bacterial communities, although the effects on pathogen suppression and tomato plant growth were comparable between treatments. The existence of pathogen-suppressive microbes, other than Clostridia previously reported to have an effect, was suggested. Comparison between RSD and SS provides new aspects of unknown disinfestation patterns and the usefulness of SS as an alternative to RSD.
KW - Bacterial communities
KW - Fusarium oxysporum
KW - Reductive soil infestation
KW - Soil solariza-tion
KW - Tomato
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U2 - 10.3390/agronomy11071375
DO - 10.3390/agronomy11071375
M3 - Article
AN - SCOPUS:85111066755
SN - 2073-4395
VL - 11
JO - Agronomy
JF - Agronomy
IS - 7
M1 - 1375
ER -