Two-dimensional tracking of a motile micro-organism allowing high-resolution observation with various imaging techniques

H. Oku, N. Ogawa, M. Ishikawa, K. Hashimoto

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

In this article, a micro-organism tracking system using a high-speed vision system is reported. This system two dimensionally tracks a freely swimming micro-organism within the field of an optical microscope by moving a chamber of target micro-organisms based on high-speed visual feedback. The system we developed could track a paramecium using various imaging techniques, including bright-field illumination, dark-field illumination, and differential interference contrast, at magnifications of 5 times and 20 times. A maximum tracking duration of 300 s was demonstrated. Also, the system could track an object with a velocity of up to 35 000 μms (175 diameterss), which is significantly faster than swimming micro-organisms.

Original languageEnglish
Article number034301
JournalReview of Scientific Instruments
Volume76
Issue number3
DOIs
Publication statusPublished - 2005 Mar 1

ASJC Scopus subject areas

  • Instrumentation

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