Two different types of dehalogenases, LinA and LinB, involved in γ- hexachlorocyclohexane degradation in Sphingomonas paucimobilis UT26 are localized in the periplasmic space without molecular processing

Yuji Nagata, Akiko Futamura, Keisuke Miyauchi, Masamichi Takagi

Research output: Contribution to journalArticlepeer-review

64 Citations (Scopus)

Abstract

γ-Hexachlorocyclohexane (γ-HCH) is one of several highly chlorinated insecticides that cause serious environmental problems. The cellular proteins of a γ-HCH-degrading bacterium, Sphingomonas paucimobilis UT26, were fractionated into periplasmic, cytosolic, and membrane fractions after osmotic shock. Most of two different types of dehalogenase, LinA (γ- hexachlorocyclohexane dehydrochlorinase) and LinB (1,3,4,6-tetrachloro-1,4- cyclohexadiene halidohydrolase), that are involved in the early steps of γ- HCH degradation in UT26 was detected in the periplasmic fraction and had not undertaken molecular processing. Furthermore, immunoelectron microscopy clearly showed that LinA and LinB are periplasmic proteins. LinA and LinB both lack a typical signal sequence for export, so they may be secreted into the periplasmic space via a hitherto unknown mechanism.

Original languageEnglish
Pages (from-to)5409-5413
Number of pages5
JournalJournal of bacteriology
Volume181
Issue number17
DOIs
Publication statusPublished - 1999 Sep

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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