Two CYP3A-like genes in the marine mussel Mytilus edulis: MRNA expression modulation following short-term exposure to endocrine disruptors

Elena Cubero-Leon, A. Mirel Puinean, Pierre Labadie, Corina Ciocan, Naoki Itoh, Mitsuyo Kishida, Makoto Osada, Christophe Minier, Elizabeth M. Hill, Jeanette M. Rotchell

Research output: Contribution to journalArticlepeer-review

25 Citations (Scopus)

Abstract

Members of the vertebrate CYP3A subfamily are involved in the metabolism of steroids and a wide range of xenobiotics. In this study two CYP3A- like mRNAs have been isolated from the mussel (Mytilus edulis), and their seasonal expression profile and modulation by estrogens examined. Sexual dimorphism of CYP3A- like mRNA expression was not observed in mussel gonads of individuals collected throughout a year. Nevertheless, natural variation in gonadal CYP3A- like mRNA expression was observed, with highest levels of CYP3A isoform1 and lowest levels of CYP3A isoform2 mRNA during the maturation and spawning season. Exposure to a 10% sewage treatment works extract did not result in any significant changes in mRNA expression of CYP3A-like. In contrast, exposure to E2 (200ng/L) and TBT (100ng/L) significantly down-regulated the expression of CYP3A- like isoform1 but not CYP3A-like isoform2 suggesting differential regulation. Understanding xenobiotic metabolism pathways in aquatic invertebrates.

Original languageEnglish
Pages (from-to)32-39
Number of pages8
JournalMarine Environmental Research
Volume74
DOIs
Publication statusPublished - 2012 Mar

Keywords

  • CYP3A
  • Ecotoxicology
  • Mussel
  • Pollution

ASJC Scopus subject areas

  • Oceanography
  • Aquatic Science
  • Pollution

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