Two-compartments microbioreactor with integrated magnetic stirrer pump for measurement of transmembrane transport of Caco-2 CELLS

H. Kimura, H. Sakai, Serge Jacques Pierre Ostrovidow, T. Yamamoto, Y. Sakai, T. Fujii

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    Abstract

    We have developed and experimentally demonstrated a microbioreactor embedded with stirrer-type micropumps for on-chip perfusion of culture medium and optical fiber connectors for on-chip and real-time fluorescent detection. The cell culture chamber was separated two compartments by a polyester-made semi-permeable membrane, which can realize polarized cell culture condition and detection of fluorescent movement in-between the two-compartments. As an evaluation of the microbioreactor, it was measured that Rhodamine-123 movement by the activity of P-glycoprotein of a small intestine model cell of Caco-2. As a result, the change of fluorescent intensity of Rhodamine-123 from basolateral side to apical side of Caco-2 cells was successfully detected.

    Original languageEnglish
    Title of host publicationProceedings of 2006 International Conference on Microtechnologies in Medicine and Biology
    Pages112-115
    Number of pages4
    DOIs
    Publication statusPublished - 2006 Dec 1
    Event2006 International Conference on Microtechnologies in Medicine and Biology - Okinawa, Japan
    Duration: 2006 May 92006 May 12

    Publication series

    NameProceedings of 2006 International Conference on Microtechnologies in Medicine and Biology

    Other

    Other2006 International Conference on Microtechnologies in Medicine and Biology
    CountryJapan
    CityOkinawa
    Period06/5/906/5/12

    Keywords

    • Caco-2
    • Magnetic stirrer pump
    • Microbioreactor
    • P-glycoprotein
    • PDMS

    ASJC Scopus subject areas

    • Biomedical Engineering
    • Electrical and Electronic Engineering
    • Medicine(all)

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  • Cite this

    Kimura, H., Sakai, H., Ostrovidow, S. J. P., Yamamoto, T., Sakai, Y., & Fujii, T. (2006). Two-compartments microbioreactor with integrated magnetic stirrer pump for measurement of transmembrane transport of Caco-2 CELLS. In Proceedings of 2006 International Conference on Microtechnologies in Medicine and Biology (pp. 112-115). [4281322] (Proceedings of 2006 International Conference on Microtechnologies in Medicine and Biology). https://doi.org/10.1109/MMB.2006.251504