Two-compartments microbioreactor with integrated magnetic stirrer pump for measurement of transmembrane transport of Caco-2 CELLS

H. Kimura, H. Sakai, Serge Jacques Pierre Ostrovidow, T. Yamamoto, Y. Sakai, T. Fujii

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    Abstract

    We have developed and experimentally demonstrated a microbioreactor embedded with stirrer-type micropumps for on-chip perfusion of culture medium and optical fiber connectors for on-chip and real-time fluorescent detection. The cell culture chamber was separated two compartments by a polyester-made semi-permeable membrane, which can realize polarized cell culture condition and detection of fluorescent movement in-between the two-compartments. As an evaluation of the microbioreactor, it was measured that Rhodamine-123 movement by the activity of P-glycoprotein of a small intestine model cell of Caco-2. As a result, the change of fluorescent intensity of Rhodamine-123 from basolateral side to apical side of Caco-2 cells was successfully detected.

    Original languageEnglish
    Title of host publicationProceedings of 2006 International Conference on Microtechnologies in Medicine and Biology
    Pages112-115
    Number of pages4
    DOIs
    Publication statusPublished - 2006 Dec 1
    Event2006 International Conference on Microtechnologies in Medicine and Biology - Okinawa, Japan
    Duration: 2006 May 92006 May 12

    Publication series

    NameProceedings of 2006 International Conference on Microtechnologies in Medicine and Biology

    Other

    Other2006 International Conference on Microtechnologies in Medicine and Biology
    Country/TerritoryJapan
    CityOkinawa
    Period06/5/906/5/12

    Keywords

    • Caco-2
    • Magnetic stirrer pump
    • Microbioreactor
    • P-glycoprotein
    • PDMS

    ASJC Scopus subject areas

    • Biomedical Engineering
    • Electrical and Electronic Engineering
    • Medicine(all)

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