Two-colour serial femtosecond crystallography dataset from gadoteridol-derivatized lysozyme for MAD phasing

Alexander Gorel, Koji Motomura, Hironobu Fukuzawa, R. Bruce Doak, Marie Luise Grünbein, Mario Hilpert, Ichiro Inoue, Marco Kloos, Gabriela Nass Kovács, Eriko Nango, Karol Nass, Christopher M. Roome, Robert L. Shoeman, Rie Tanaka, Kensuke Tono, Lutz Foucar, Yasumasa Joti, Makina Yabashi, So Iwata, Kiyoshi UedaThomas R.M. Barends, Ilme Schlichting

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

We provide a detailed description of a gadoteridol-derivatized lysozyme (gadolinium lysozyme) two-colour serial femtosecond crystallography (SFX) dataset for multiple wavelength anomalous dispersion (MAD) structure determination. The data was collected at the Spring-8 Angstrom Compact free-electron LAser (SACLA) facility using a two-colour double-pulse beam to record two diffraction patterns simultaneously in one diffraction image. Gadolinium lysozyme was chosen as a well-established model system that has a very strong anomalous signal. Diffraction patterns from gadolinium lysozyme microcrystals were recorded to a resolution of 1.9 Å in both colours. This dataset is publicly available through the Coherent X-ray Imaging Data Bank (CXIDB) as a resource for algorithm development.

Original languageEnglish
Article number170188
JournalScientific Data
Volume4
DOIs
Publication statusPublished - 2017 Dec 12

ASJC Scopus subject areas

  • Statistics and Probability
  • Information Systems
  • Education
  • Computer Science Applications
  • Statistics, Probability and Uncertainty
  • Library and Information Sciences

Fingerprint Dive into the research topics of 'Two-colour serial femtosecond crystallography dataset from gadoteridol-derivatized lysozyme for MAD phasing'. Together they form a unique fingerprint.

  • Cite this

    Gorel, A., Motomura, K., Fukuzawa, H., Doak, R. B., Grünbein, M. L., Hilpert, M., Inoue, I., Kloos, M., Nass Kovács, G., Nango, E., Nass, K., Roome, C. M., Shoeman, R. L., Tanaka, R., Tono, K., Foucar, L., Joti, Y., Yabashi, M., Iwata, S., ... Schlichting, I. (2017). Two-colour serial femtosecond crystallography dataset from gadoteridol-derivatized lysozyme for MAD phasing. Scientific Data, 4, [170188]. https://doi.org/10.1038/sdata.2017.188