TY - JOUR
T1 - Twist induces reversal of myotube formation
AU - Hjiantoniou, Eleni
AU - Anayasa, Mustafa
AU - Nicolaou, Paschalis
AU - Bantounas, Ioannis
AU - Saito, Masahiro
AU - Iseki, Sachiko
AU - Uney, James B.
AU - Phylactou, Leonidas A.
N1 - Funding Information:
Fig. 5 Reduction of myogenic markers MyoD, myogenin, and MHC followed by re-entry to the cell cycle and accompanied by myotube cleavage. (A) Overexpression of the Twist cDNA (AdT) caused an increase in BrdU-positive cells as shown with the intense nuclear coloring compared with untransfected (C2C12) and control-transfected cells (AdC). (B) Reduction of myogenic markers MyoD, myogenin, and MHC occurred after transfection of myotubes with the AdT viral vector, as detected by triple immunocytochemistry, coupled with BrdU activity, 3 days after induction with Acknowledgment This work has been supported by the A.G. Leventis Foundation (grant to L. A. P.) and the Cyprus Research Promotion Foundation (grant to L. A. P.).
PY - 2008/2
Y1 - 2008/2
N2 - Mammals possess reduced ability to regenerate lost tissue, compared with other vertebrates, which can regenerate through differentiation of precursor cells or de-differentiation. Mammalian multinucleated myotube formation is a differentiation process, which arises from the fusion of mononucleated myoblasts and is thought to be an irreversible process toward muscle formation. By overexpressing the Twist gene in terminally differentiated myotubes, we managed to induce reversal of cell differentiation. More specifically, following expression of the Twist gene, myotubes underwent morphological changes that caused them to cleave. This was accompanied by a reduction in the expression of certain myogenic markers. Interestingly, Twist overexpression also caused a reduction in the muscle transcription factor MyoD. Further experiments showed an increase in the cell cycle entry molecule, cyclin D1 and initiation of DNA synthesis, due to Twist overexpression. The exploitation of Twist-mediated reversal of differentiation and the study of its specific mechanism would be important in order to study mammalian cellular de-differentiation and determine its potential in muscle regeneration.
AB - Mammals possess reduced ability to regenerate lost tissue, compared with other vertebrates, which can regenerate through differentiation of precursor cells or de-differentiation. Mammalian multinucleated myotube formation is a differentiation process, which arises from the fusion of mononucleated myoblasts and is thought to be an irreversible process toward muscle formation. By overexpressing the Twist gene in terminally differentiated myotubes, we managed to induce reversal of cell differentiation. More specifically, following expression of the Twist gene, myotubes underwent morphological changes that caused them to cleave. This was accompanied by a reduction in the expression of certain myogenic markers. Interestingly, Twist overexpression also caused a reduction in the muscle transcription factor MyoD. Further experiments showed an increase in the cell cycle entry molecule, cyclin D1 and initiation of DNA synthesis, due to Twist overexpression. The exploitation of Twist-mediated reversal of differentiation and the study of its specific mechanism would be important in order to study mammalian cellular de-differentiation and determine its potential in muscle regeneration.
KW - Myotubes
KW - Reversal of differentiation
KW - Twist
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U2 - 10.1111/j.1432-0436.2007.00195.x
DO - 10.1111/j.1432-0436.2007.00195.x
M3 - Article
C2 - 17662069
AN - SCOPUS:38849084613
VL - 76
SP - 182
EP - 192
JO - Differentiation
JF - Differentiation
SN - 0301-4681
IS - 2
ER -
