Turn-on fluorescent probe with visible light excitation for labeling of hexahistidine tagged protein

Mie Kamoto, Naoki Umezawa, Nobuki Kato, Tsunehiko Higuchi

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

We report here the development of a novel fluorescein-based probe which shows selective fluorescence enhancement on binding to a hexahistidine-tagged protein. No fluorescence change was observed with untagged protein. This probe is excitable with visible light and is considered to be suitable for use in biological applications.

Original languageEnglish
Pages (from-to)2285-2288
Number of pages4
JournalBioorganic and Medicinal Chemistry Letters
Volume19
Issue number8
DOIs
Publication statusPublished - 2009 Apr 15
Externally publishedYes

Keywords

  • Fluorescent probe
  • Hexahistidine tag
  • Molecular recognition

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Pharmaceutical Science
  • Drug Discovery
  • Clinical Biochemistry
  • Organic Chemistry

Fingerprint Dive into the research topics of 'Turn-on fluorescent probe with visible light excitation for labeling of hexahistidine tagged protein'. Together they form a unique fingerprint.

Cite this