Turn-on fluorescence switch involving aggregation and elimination processes for β-lactamase-tag

Kalyan K. Sadhu, Shin Mizukami, Shuji Watanabe, Kazuya Kikuchi

Research output: Contribution to journalArticlepeer-review

24 Citations (Scopus)

Abstract

The targeted protein of interest is fused with genetically modified β-lactamase enzyme, which reacts with the probe in physiological conditions to break the aggregated interaction between the fluorophore and quencher. This alliance-separation technique is new for protein labeling and is probed in vitro and in live cell imaging studies.

Original languageEnglish
Pages (from-to)7403-7405
Number of pages3
JournalChemical Communications
Volume46
Issue number39
DOIs
Publication statusPublished - 2010 Oct 21
Externally publishedYes

ASJC Scopus subject areas

  • Catalysis
  • Electronic, Optical and Magnetic Materials
  • Ceramics and Composites
  • Chemistry(all)
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Materials Chemistry

Fingerprint Dive into the research topics of 'Turn-on fluorescence switch involving aggregation and elimination processes for β-lactamase-tag'. Together they form a unique fingerprint.

Cite this