Trimethine cyanine dyes as deep-red fluorescent indicators with high selectivity to the internal loop of the bacterial A-site RNA

Yusuke Sato; Sayaka Yajima; Akifumi Taguchi; Kyosuke Baba; Mayu Nakagomi; Yuri Aiba; Seiichi Nishizawa

doi:10.1039/c9cc00414a

Trimethine cyanine dyes as deep-red fluorescent indicators with high selectivity to the internal loop of the bacterial A-site RNA

Yusuke Sato, Sayaka Yajima, Akifumi Taguchi, Kyosuke Baba, Mayu Nakagomi, Yuri Aiba, Seiichi Nishizawa

SCI - Chemistry

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

We report that TO-PRO-3, a thiazole orange analogue with a trimethine bridge, functions as a deep-red fluorescent indicator for the internal loop structure of the bacterial (Escherichia coli) ribosomal decoding region of the aminoacyl-tRNA site (A-site), which enables the assessment of A-site binding capability of various test compounds including blue and even-green-emitting compounds.

Original language	English
Pages (from-to)	3183-3186
Number of pages	4
Journal	Chemical Communications
Volume	55
Issue number	22
DOIs	https://doi.org/10.1039/c9cc00414a
Publication status	Published - 2019

ASJC Scopus subject areas

Catalysis
Electronic, Optical and Magnetic Materials
Ceramics and Composites
Chemistry(all)
Surfaces, Coatings and Films
Metals and Alloys
Materials Chemistry

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title = "Trimethine cyanine dyes as deep-red fluorescent indicators with high selectivity to the internal loop of the bacterial A-site RNA",

abstract = "We report that TO-PRO-3, a thiazole orange analogue with a trimethine bridge, functions as a deep-red fluorescent indicator for the internal loop structure of the bacterial (Escherichia coli) ribosomal decoding region of the aminoacyl-tRNA site (A-site), which enables the assessment of A-site binding capability of various test compounds including blue and even-green-emitting compounds.",

author = "Yusuke Sato and Sayaka Yajima and Akifumi Taguchi and Kyosuke Baba and Mayu Nakagomi and Yuri Aiba and Seiichi Nishizawa",

note = "Funding Information: This work was supported by Grant-in-Aid for Scientific Research (B) (No. 16H04159) and Challenging Exploratory Research (No. 17K19133) from Japan Society for the Promotion of Science (JSPS). Publisher Copyright: {\textcopyright} 2019 The Royal Society of Chemistry.",

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T1 - Trimethine cyanine dyes as deep-red fluorescent indicators with high selectivity to the internal loop of the bacterial A-site RNA

AU - Sato, Yusuke

AU - Yajima, Sayaka

AU - Taguchi, Akifumi

AU - Baba, Kyosuke

AU - Nakagomi, Mayu

AU - Aiba, Yuri

AU - Nishizawa, Seiichi

N1 - Funding Information: This work was supported by Grant-in-Aid for Scientific Research (B) (No. 16H04159) and Challenging Exploratory Research (No. 17K19133) from Japan Society for the Promotion of Science (JSPS). Publisher Copyright: © 2019 The Royal Society of Chemistry.

PY - 2019

Y1 - 2019

N2 - We report that TO-PRO-3, a thiazole orange analogue with a trimethine bridge, functions as a deep-red fluorescent indicator for the internal loop structure of the bacterial (Escherichia coli) ribosomal decoding region of the aminoacyl-tRNA site (A-site), which enables the assessment of A-site binding capability of various test compounds including blue and even-green-emitting compounds.

AB - We report that TO-PRO-3, a thiazole orange analogue with a trimethine bridge, functions as a deep-red fluorescent indicator for the internal loop structure of the bacterial (Escherichia coli) ribosomal decoding region of the aminoacyl-tRNA site (A-site), which enables the assessment of A-site binding capability of various test compounds including blue and even-green-emitting compounds.

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VL - 55

SP - 3183

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JO - Chemical Communications

JF - Chemical Communications

SN - 1359-7345

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ER -

Trimethine cyanine dyes as deep-red fluorescent indicators with high selectivity to the internal loop of the bacterial A-site RNA

Abstract

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