Triggering of the vascular permeability reaction by activation of the Hageman factor-prekallikrein system by house dust mite proteinase

Keishi Maruo, Takaaki Akaike, Yasuhiro Matsumura, Shoichi Kohmoto, Yuji Inada, Tomomichi Ono, Tatsuyoshi Arao, Hiroshi Maeda

Research output: Contribution to journalArticlepeer-review

31 Citations (Scopus)

Abstract

A 30-kilodalton (kDa) proteinase from the house dust mite Dermatophagoides farinae (Df-proteinase) was recently purified (Takahashi et al. (1990) Int. Arch. Allergy Appl. Immunol. 91, 80-85). In this paper we detailed the biological activities of the Df-proteinase. The activation of the kinin cascade by Df-proteinase was examined in vitro by using purified guinea pig Hageman factor (HF), prekallikrein (PK) and high-molecular-weight kininogen (HMWK) and the effect of this proteinase on endogenous human plasma proteinase inhibitors (serpins) and α2-macroglobulin was tested. In addition, enhancement of the vascular permeability reaction in guinea pig skin by Df-proteinase was examined in vivo. These experiments showed that Df-proteinase could activate all the steps of the kinin-generating cascade, i.e., HF, PK and HMWK, and that Df-proteinase retained proteolytic activity even in the presence of an excess amount of endogenous proteinase inhibitors in plasma. We also found that the marked enhancement of the vascular permeability reaction was induced by Df-proteinase via the activation of the kinin-generating cascade without the release of histamine. From these results, we conclude that the proteinase of the house dust mite, Df-proteinase, has the potential to generate bradykinin and that the presence of this proteinase in biological systems would exacerbate inflammatory reactions in some pathological conditions.

Original languageEnglish
Pages (from-to)62-68
Number of pages7
JournalBBA - General Subjects
Volume1074
Issue number1
DOIs
Publication statusPublished - 1991 May 24
Externally publishedYes

Keywords

  • (House dust mite)
  • Mite proteinase
  • Proteinase inhibitor
  • Vascular permeability reaction

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology

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