Tri-n-butyltin-induced blockade of store-operated calcium influx in rat thymocytes

Aimi Kanada, Jun ya Yamaguchi, Yumiko Nishimura, Tomohiro M. Oyama, Masaya Satoh, Norihiro Katayama, Ken ichi Chikutei, Yoshiro Okano, Yasuo Oyama

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Tri-n-butyltin (TBT), one of environmental pollutants, disturbs intracellular Ca2+ homeostasis by increasing intracellular Ca2+ concentration ([Ca2+]i). Effect of TBT on oscillatory change in [Ca2+]i (Ca2+ oscillation) of rat thymocytes was examined using a laser microscope with fluo-3-AM in order to further elucidate the TBT toxicity related to intracellular Ca2+. The Ca2+ oscillation was completely attenuated by 300 nM TBT. Since store-operated Ca2+ channels are involved in the generation of Ca2+ oscillation, the action of TBT on an increase in [Ca2+]i by Ca2+ influx through store-operated Ca2+ channels was examined. The increase in [Ca2+]i by the store-operated Ca2+ influx was not affected by 3 nM TBT. However, TBT at 10 nM or more significantly reduced the increase in [Ca2+]i. It is likely that TBT attenuates the Ca2+ oscillation by reducing the Ca2+ influx through store-operated Ca2+ channels.

Original languageEnglish
Pages (from-to)213-218
Number of pages6
JournalEnvironmental Toxicology and Pharmacology
Volume22
Issue number2
DOIs
Publication statusPublished - 2006 Sep 1
Externally publishedYes

Keywords

  • Ca oscillation
  • Store-operated Ca channel
  • Tri-n-butyltin

ASJC Scopus subject areas

  • Toxicology
  • Pharmacology
  • Health, Toxicology and Mutagenesis

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  • Cite this

    Kanada, A., Yamaguchi, J. Y., Nishimura, Y., Oyama, T. M., Satoh, M., Katayama, N., Chikutei, K. I., Okano, Y., & Oyama, Y. (2006). Tri-n-butyltin-induced blockade of store-operated calcium influx in rat thymocytes. Environmental Toxicology and Pharmacology, 22(2), 213-218. https://doi.org/10.1016/j.etap.2006.03.007