TY - JOUR
T1 - Tri-n-butyltin-induced blockade of store-operated calcium influx in rat thymocytes
AU - Kanada, Aimi
AU - Yamaguchi, Jun ya
AU - Nishimura, Yumiko
AU - Oyama, Tomohiro M.
AU - Satoh, Masaya
AU - Katayama, Norihiro
AU - Chikutei, Ken ichi
AU - Okano, Yoshiro
AU - Oyama, Yasuo
PY - 2006/9/1
Y1 - 2006/9/1
N2 - Tri-n-butyltin (TBT), one of environmental pollutants, disturbs intracellular Ca2+ homeostasis by increasing intracellular Ca2+ concentration ([Ca2+]i). Effect of TBT on oscillatory change in [Ca2+]i (Ca2+ oscillation) of rat thymocytes was examined using a laser microscope with fluo-3-AM in order to further elucidate the TBT toxicity related to intracellular Ca2+. The Ca2+ oscillation was completely attenuated by 300 nM TBT. Since store-operated Ca2+ channels are involved in the generation of Ca2+ oscillation, the action of TBT on an increase in [Ca2+]i by Ca2+ influx through store-operated Ca2+ channels was examined. The increase in [Ca2+]i by the store-operated Ca2+ influx was not affected by 3 nM TBT. However, TBT at 10 nM or more significantly reduced the increase in [Ca2+]i. It is likely that TBT attenuates the Ca2+ oscillation by reducing the Ca2+ influx through store-operated Ca2+ channels.
AB - Tri-n-butyltin (TBT), one of environmental pollutants, disturbs intracellular Ca2+ homeostasis by increasing intracellular Ca2+ concentration ([Ca2+]i). Effect of TBT on oscillatory change in [Ca2+]i (Ca2+ oscillation) of rat thymocytes was examined using a laser microscope with fluo-3-AM in order to further elucidate the TBT toxicity related to intracellular Ca2+. The Ca2+ oscillation was completely attenuated by 300 nM TBT. Since store-operated Ca2+ channels are involved in the generation of Ca2+ oscillation, the action of TBT on an increase in [Ca2+]i by Ca2+ influx through store-operated Ca2+ channels was examined. The increase in [Ca2+]i by the store-operated Ca2+ influx was not affected by 3 nM TBT. However, TBT at 10 nM or more significantly reduced the increase in [Ca2+]i. It is likely that TBT attenuates the Ca2+ oscillation by reducing the Ca2+ influx through store-operated Ca2+ channels.
KW - Ca oscillation
KW - Store-operated Ca channel
KW - Tri-n-butyltin
UR - http://www.scopus.com/inward/record.url?scp=33745932947&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33745932947&partnerID=8YFLogxK
U2 - 10.1016/j.etap.2006.03.007
DO - 10.1016/j.etap.2006.03.007
M3 - Article
C2 - 21783712
AN - SCOPUS:33745932947
VL - 22
SP - 213
EP - 218
JO - Environmental Toxicology and Pharmacology
JF - Environmental Toxicology and Pharmacology
SN - 1382-6689
IS - 2
ER -