Trehalose enhanced the freezability of Poodle dog sperm collected by an artificial vagina (AV)

Hideaki Yamashiro, Kaori Narita, Satoshi Sugimura, Young Joon Han, Atsushi Sugawara, Kanako Morohaku, Fumiaki Nakazato, Tsutomu Konno, Muneyoshi Yoshida, Eimei Sato

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

In an attempt to develop a suitable freezing method for Poodle dog sperm, an experiment was conducted to investigate semen collection methods of digital stimulation and an artificial vagina (AV), using Tris and trehalose-egg yolk extender, on the characteristics and cryopreservation of sperm. Two dogs (dogs A and B) were subjected to semen collection by digital stimulation and AV. The volume, sperm concentration, sperm motility index (SMI) and acrosome status of ejaculates were determined immediately after collection. The remainder was frozen as pellets in Tris and trehalose-egg yolk extender. Sperm motility index was evaluated after thawing and during a thermal resistance test, and acrosome integrity was also assessed. No significant differences regarding sperm concentrations, SMI and acrosome integrity were observed between semen collected by AV and digital stimulation. However, when dog sperm were collected by an AV and frozen in trehalose-egg yolk extender, the motility index of frozen-thawed sperm was significantly improved compared to sperm frozen in Tris-egg yolk extender which were collected by digital stimulation. In conclusion, semen collected by an AV and frozen in trehalose-egg yolk extender was effective in enhancing the freezability of Poodle dog sperm.

Original languageEnglish
Pages (from-to)165-171
Number of pages7
JournalAnimal Reproduction Science
Volume102
Issue number1-2
DOIs
Publication statusPublished - 2007 Nov 1

Keywords

  • Dog artificial vagina
  • Freezability
  • Poodle dog sperm
  • Trehalose

ASJC Scopus subject areas

  • Food Animals
  • Animal Science and Zoology
  • Endocrinology

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    Yamashiro, H., Narita, K., Sugimura, S., Han, Y. J., Sugawara, A., Morohaku, K., Nakazato, F., Konno, T., Yoshida, M., & Sato, E. (2007). Trehalose enhanced the freezability of Poodle dog sperm collected by an artificial vagina (AV). Animal Reproduction Science, 102(1-2), 165-171. https://doi.org/10.1016/j.anireprosci.2007.02.026