Treatment of low-strength wastewater in an anaerobic down-flow hanging sponge (AnDHS) reactor at low temperature

Haruhiko Sumino, Ryuichi Murota, Ai Miyashita, Hiroyuki Imachi, Akiyoshi Ohashi, Hideki Harada, Kazuaki Syutsubo

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)


The process performance of a novel anaerobic down-flow hanging sponge (AnDHS) reactor for the treatment of low strength wastewater was investigated. A lab-scale experiment was conducted in which 300-400 mgCOD L-1 of artificial wastewater was fed in over 600days. The reactor exhibited sufficient performance: 70-90% of total COD removal, and 60-90% of methane recovery were maintained at 20°C, with a hydraulic retention time (HRT) of 2h. It was possible to maintain COD removal by extending the HRT to 4h at 15°C and 10h at 10°C. With regard to the wastewater feed, one-pass mode (without effluent recirculation) gave better performance in COD removal as compared with recirculation mode. The results of batch feeding experiments using single substrates (such as acetate, propionate or sucrose) indicated that acetate degradation was more strongly affected by decreasing operational temperature. In addition, the AnDHS reactor system had no significant problems related to sludge retention such as massive loss of sludge throughout the experiment. Microbial structure analysis of the retained sludge with respect to the domain Archaeal 16S rRNA gene showed the proliferation of relatives of both the acetate-utilizing genus Methanosaeta and the hydrogen-utilizing genus Methanolinea.

Original languageEnglish
Pages (from-to)1803-1808
Number of pages6
JournalJournal of Environmental Science and Health - Part A Toxic/Hazardous Substances and Environmental Engineering
Issue number12
Publication statusPublished - 2012 Oct 1


  • AnDHS
  • Anaerobic treatment
  • effluent recirculation
  • low strength
  • low temperature
  • methanogen

ASJC Scopus subject areas

  • Environmental Engineering


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