Tre1 GPCR initiates germ cell transepithelial migration by regulating Drosophila melanogaster E-cadherin

Prabhat S. Kunwar, Hiroko Sano, Andrew D. Renault, Vitor Barbosa, Naoyuki Fuse, Ruth Lehmann

Research output: Contribution to journalArticlepeer-review

54 Citations (Scopus)

Abstract

Despite significant progress in identifying the guidance pathways that control cell migration, how a cell starts to move within an intact organism, acquires motility, and loses contact with its neighbors is poorly understood. We show that activation of the G protein-coupled receptor (GPCR) trapped in endoderm 1 (Tre1) directs the redistribution of the G protein Gβ as well as adherens junction proteins and Rho guanosine triphosphatase from the cell periphery to the lagging tail of germ cells at the onset of Drosophila melanogaster germ cell migration. Subsequently, Tre1 activity triggers germ cell dispersal and orients them toward the midgut for directed transepithelial migration. A transition toward invasive migration is also a prerequisite for metastasis formation, which often correlates with down-regulation of adhesion proteins. We show that uniform down-regulation of E-cadherin causes germ cell dispersal but is not sufficient for transepithelial migration in the absence of Tre1. Our findings therefore suggest a new mechanism for GPCR function that links cell polarity, modulation of cell adhesion, and invasion.

Original languageEnglish
Pages (from-to)157-168
Number of pages12
JournalJournal of Cell Biology
Volume183
Issue number1
DOIs
Publication statusPublished - 2008 Oct 6

ASJC Scopus subject areas

  • Cell Biology

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