TRAV7-2*02 expressing CD8+ T cells are responsible for palladium allergy

Yuri Takeda, Yoshiko Suto, Koyu Ito, Wataru Hshimoto, Tadashi Nishiya, Kyosuke Ueda, Takayuki Narushima, Tetsu Takahashi, Kouetsu Ogasawara

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

While metallic biomaterials have led to an improvement in the quality of life, metal allergies, especially to palladium (Pd), has caused a recent increase in allergic patients. Metal allergy is known to be a T cell-mediated delayed-type hypersensitivity (DTH); however, the pathogenic T cell subsets and the specific T cell receptor (TCR) have not been identified. Therefore, we attempted to identify the pathogenic T cells responsible for Pd allergy. We found that activating CD8+ T cells significantly increased and that the TRAV (TCRα variable) 7-2*02 chain skewed in Pd allergic mice. Furthermore, adoptive transfer experiments revealed that in vitro-cultured Pd-stimulated antigen presenting cells (APCs) function as memory APCs with recipient mice developing Pd allergy and that the frequency of TRAV7-2*02 increases the same as conventional Pd allergic mice. In contrast, neither proliferation of CD8+ T cells nor increasing of TRAV7-2*02 was observed in major histocompatibility complex I (MHC I)-deficient Pd-APCs transferred to mice. Taken together, we revealed that TRAV7-2*02-expressing CD8+ T cells are the pathogenic T cells for the development of Pd allergy. We also identified the CDR3 consensus motif of pathogenic TCRs as CAAXSGSWQLIF in TRAV7-2*02/TRAJ (TCRα junction)22*01 positive cells. These results suggest that the specific TCRs represent novel targets for the development of diagnostics and treatments for metal allergy.

Original languageEnglish
Article number1162
JournalInternational journal of molecular sciences
Volume18
Issue number6
DOIs
Publication statusPublished - 2017 Jun

Keywords

  • Animal models
  • Autoimmunity
  • Biomaterial(s)
  • Metal allergy
  • T cell receptor (TCR)
  • T cells

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

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