Transscleral controlled delivery of geranylgeranylaceton using a polymeric device protects rat retina against light injury

Research output: Chapter in Book/Report/Conference proceedingChapter

5 Citations (Scopus)

Abstract

We evaluated the effects of a transscleral drug delivery device, consisting of a reservoir and controlled-release cover, which were made of photopolymerized polyethylene glycol dimethacrylate and triethylene glycol dimethacrylate, combined at different ratios. Geranylgeranylacetone (GGA), a heat-shock protein (HSP) inducer, was loaded into the device. The GGA was released from the device under zero-order kinetics. At both 1 week and 4 weeks after device implantation on rat sclera, HSP70 gene and protein expression were up-regulated in the sclerachoroid- retinal pigment epithelium fraction of rat eyes treated with the GGA-loaded device compared with rat eyes treated with saline-loaded devices or eyes of nontreated rats. Flash electroretinograms were recorded 4 days after white light exposure (8000 lx for 18 h). Electroretinographic amplitudes of the a- and b-waves were preserved significantly in rats treated with GGA-loaded devices compared with rats treated with saline-loaded devices. Histological examination showed that the outer nuclear layer thickness was preserved in rats that had the GGA-loaded device. These results may show that transscleral GGA delivery using our device may offer an alternative method to treat retinal diseases.

Original languageEnglish
Title of host publicationAdvances in Experimental Medicine and Biology
PublisherSpringer New York LLC
Pages471-477
Number of pages7
DOIs
Publication statusPublished - 2016 Oct 1

Publication series

NameAdvances in Experimental Medicine and Biology
Volume854
ISSN (Print)0065-2598
ISSN (Electronic)2214-8019

Keywords

  • Drug delivery
  • Geranylgeranylacetone
  • Heat shock protein (HSP)
  • Phototoxicity
  • Poly(ethyleneglycol) dimethacrylate (PEG)
  • Transsclera
  • Tri(ethyleneglycol) dimethacrylate (TEG)

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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  • Cite this

    Nagai, N., Kaji, H., Nishizawa, M., Nakazawa, T., & Abe, T. (2016). Transscleral controlled delivery of geranylgeranylaceton using a polymeric device protects rat retina against light injury. In Advances in Experimental Medicine and Biology (pp. 471-477). (Advances in Experimental Medicine and Biology; Vol. 854). Springer New York LLC. https://doi.org/10.1007/978-3-319-17121-0_63