Transplantation of preserved pancreatic islets into the portal vein of rats

Gunji Yamazaki, Gizo Nakagawara, Hiroshi Okamoto, Ryuichi Akimoto, Shoichi Kimura, Yasuhiko Kojima, Itsuo Miyazaki

Research output: Contribution to journalArticlepeer-review


Langerhans islets were isolated from the exocrine pancreata of Wistar rats by the improved collagenase-digestion method. The isolated islets were preserved in a tissue culture medium for seven days. Transplantation of these preserved islets into the portal vein of streptozotocin-induced diabetic rats resulted in a significant reduction of hyperglycemia, polyuria and glucosuria, and a restoration of weight gain. It was found that these effects could be maintained for 16 weeks. In order to normalize the K-values and plasma insulin levels, at least 600 islets had to be transplanted into each diabetic rat.

Original languageEnglish
Pages (from-to)152-158
Number of pages7
JournalThe Japanese Journal of Surgery
Issue number2
Publication statusPublished - 1978 Jun 1
Externally publishedYes


  • collagenase-digestion
  • diabetes mellitus
  • glucose tolerance test
  • in vitro preservation
  • insulin release
  • isolation of pancreatic islets
  • transplantation

ASJC Scopus subject areas

  • Surgery


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