Abstract
Langerhans islets were isolated from the exocrine pancreata of Wistar rats by the improved collagenase-digestion method. The isolated islets were preserved in a tissue culture medium for seven days. Transplantation of these preserved islets into the portal vein of streptozotocin-induced diabetic rats resulted in a significant reduction of hyperglycemia, polyuria and glucosuria, and a restoration of weight gain. It was found that these effects could be maintained for 16 weeks. In order to normalize the K-values and plasma insulin levels, at least 600 islets had to be transplanted into each diabetic rat.
| Original language | English |
|---|---|
| Pages (from-to) | 152-158 |
| Number of pages | 7 |
| Journal | The Japanese Journal of Surgery |
| Volume | 8 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 1978 Jun 1 |
| Externally published | Yes |
Keywords
- collagenase-digestion
- diabetes mellitus
- glucose tolerance test
- in vitro preservation
- insulin release
- isolation of pancreatic islets
- transplantation
ASJC Scopus subject areas
- Surgery
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Cite this
Yamazaki, G., Nakagawara, G., Okamoto, H., Akimoto, R., Kimura, S., Kojima, Y., & Miyazaki, I. (1978). Transplantation of preserved pancreatic islets into the portal vein of rats. The Japanese Journal of Surgery, 8(2), 152-158. https://doi.org/10.1007/BF02469371