Transplantation of preserved pancreatic islets into the portal vein of rats

Gunji Yamazaki; Gizo Nakagawara; Hiroshi Okamoto; Ryuichi Akimoto; Shoichi Kimura; Yasuhiko Kojima; Itsuo Miyazaki

doi:10.1007/BF02469371

Transplantation of preserved pancreatic islets into the portal vein of rats

Gunji Yamazaki, Gizo Nakagawara, Hiroshi Okamoto, Ryuichi Akimoto, Shoichi Kimura, Yasuhiko Kojima, Itsuo Miyazaki

Research output: Contribution to journal › Article › peer-review

Abstract

Langerhans islets were isolated from the exocrine pancreata of Wistar rats by the improved collagenase-digestion method. The isolated islets were preserved in a tissue culture medium for seven days. Transplantation of these preserved islets into the portal vein of streptozotocin-induced diabetic rats resulted in a significant reduction of hyperglycemia, polyuria and glucosuria, and a restoration of weight gain. It was found that these effects could be maintained for 16 weeks. In order to normalize the K-values and plasma insulin levels, at least 600 islets had to be transplanted into each diabetic rat.

Original language	English
Pages (from-to)	152-158
Number of pages	7
Journal	The Japanese Journal of Surgery
Volume	8
Issue number	2
DOIs	https://doi.org/10.1007/BF02469371
Publication status	Published - 1978 Jun 1
Externally published	Yes

Keywords

collagenase-digestion
diabetes mellitus
glucose tolerance test
in vitro preservation
insulin release
isolation of pancreatic islets
transplantation

ASJC Scopus subject areas

Surgery

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1007/BF02469371

Cite this

@article{ce3a810f3dc84339a67ddee988d2dc5f,

title = "Transplantation of preserved pancreatic islets into the portal vein of rats",

abstract = "Langerhans islets were isolated from the exocrine pancreata of Wistar rats by the improved collagenase-digestion method. The isolated islets were preserved in a tissue culture medium for seven days. Transplantation of these preserved islets into the portal vein of streptozotocin-induced diabetic rats resulted in a significant reduction of hyperglycemia, polyuria and glucosuria, and a restoration of weight gain. It was found that these effects could be maintained for 16 weeks. In order to normalize the K-values and plasma insulin levels, at least 600 islets had to be transplanted into each diabetic rat.",

keywords = "collagenase-digestion, diabetes mellitus, glucose tolerance test, in vitro preservation, insulin release, isolation of pancreatic islets, transplantation",

author = "Gunji Yamazaki and Gizo Nakagawara and Hiroshi Okamoto and Ryuichi Akimoto and Shoichi Kimura and Yasuhiko Kojima and Itsuo Miyazaki",

year = "1978",

month = jun,

day = "1",

doi = "10.1007/BF02469371",

language = "English",

volume = "8",

pages = "152--158",

journal = "Surgery Today",

issn = "0941-1291",

publisher = "Springer Japan",

number = "2",

}

TY - JOUR

T1 - Transplantation of preserved pancreatic islets into the portal vein of rats

AU - Yamazaki, Gunji

AU - Nakagawara, Gizo

AU - Okamoto, Hiroshi

AU - Akimoto, Ryuichi

AU - Kimura, Shoichi

AU - Kojima, Yasuhiko

AU - Miyazaki, Itsuo

PY - 1978/6/1

Y1 - 1978/6/1

N2 - Langerhans islets were isolated from the exocrine pancreata of Wistar rats by the improved collagenase-digestion method. The isolated islets were preserved in a tissue culture medium for seven days. Transplantation of these preserved islets into the portal vein of streptozotocin-induced diabetic rats resulted in a significant reduction of hyperglycemia, polyuria and glucosuria, and a restoration of weight gain. It was found that these effects could be maintained for 16 weeks. In order to normalize the K-values and plasma insulin levels, at least 600 islets had to be transplanted into each diabetic rat.

AB - Langerhans islets were isolated from the exocrine pancreata of Wistar rats by the improved collagenase-digestion method. The isolated islets were preserved in a tissue culture medium for seven days. Transplantation of these preserved islets into the portal vein of streptozotocin-induced diabetic rats resulted in a significant reduction of hyperglycemia, polyuria and glucosuria, and a restoration of weight gain. It was found that these effects could be maintained for 16 weeks. In order to normalize the K-values and plasma insulin levels, at least 600 islets had to be transplanted into each diabetic rat.

KW - collagenase-digestion

KW - diabetes mellitus

KW - glucose tolerance test

KW - in vitro preservation

KW - insulin release

KW - isolation of pancreatic islets

KW - transplantation

UR - http://www.scopus.com/inward/record.url?scp=0018081714&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0018081714&partnerID=8YFLogxK

U2 - 10.1007/BF02469371

DO - 10.1007/BF02469371

M3 - Article

C2 - 98659

AN - SCOPUS:0018081714

VL - 8

SP - 152

EP - 158

JO - Surgery Today

JF - Surgery Today

SN - 0941-1291

IS - 2

ER -

Transplantation of preserved pancreatic islets into the portal vein of rats

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this