Transparent, tough collagen laminates prepared by oriented flow casting, multi-cyclic vitrification and chemical cross-linking

Yuji Tanaka, Koichi Baba, Thomas J. Duncan, Akira Kubota, Toru Asahi, Andrew J. Quantock, Masayuki Yamato, Teruo Okano, Kohji Nishida

Research output: Contribution to journalArticlepeer-review

31 Citations (Scopus)


The lamellar architecture found in many natural fibrous tissues has a significant bearing on their specific functions. However, current engineered tissues have simultaneously no realistic structures and no adequate functions. This study demonstrates a two-step process for obtaining structurally mimicking laminates in natural fibrous tissues with good optical and mechanical characters from purified-clinically-safe collagen molecules. Stacked lamella structures can be created by repeating flow casting, with the controlling parallel/orthogonal directionalities of each thin single-layer (2-5 μm in thickness). The transparency of laminates is successfully improved by a unique multi-cyclic vitrification with chemical cross-linking. The directionalities of optical and mechanical functions in laminates are strongly related with the preferential collagen alignments in the laminates. The tensile strength of laminates is extremely higher than any other engineered materials as well as native cornea, which exhibit an orthogonal laminated collagen structure and a good optical transmission.

Original languageEnglish
Pages (from-to)3358-3366
Number of pages9
Issue number13
Publication statusPublished - 2011 May
Externally publishedYes


  • Biofilm
  • Biomimetic material
  • Collagen structure
  • Cornea
  • Fibrous tissue
  • Soft tissue biomechanics

ASJC Scopus subject areas

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials


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