Translational Research of Peritoneal Dialysis Solution with Dissolved Molecular Hydrogen

Masaaki Nakayama, Kimio Watanabe, Yoshimitsu Hayashi, Hiroyuki Terawaki, Wan Jun Zhu, Shigeru Kabayama, Sadayoshi Ito

Research output: Contribution to journalArticlepeer-review

Abstract

Background: Improved biocompatibility of peritoneal dialysis solution (PDS) is crucial for peritoneal membrane preservation, thereby ensuring long-term peritoneal dialysis (PD) and preventing encapsulating peritoneal sclerosis. We previously reported the protective effect of molecular hydrogen (H2) on mesothelial cells from PDS in nonuremic rats. Summary: In the present study, we examined the effect of H2-containing PDS (commercially available neutral pH type) regarding the protection of peritoneal tissue in experimental chronic kidney disease rats. Furthermore, we conducted a 2-week clinical trial in which H2-containing PDS was used in place of standard PDS and its feasibility was examined. In the experimental study, test solutions were injected through the subcutaneous port into the abdomen for 3 weeks. Histological study revealed a significant increase in the number of mesothelial cells and a significant decrease in peritoneal thickness in the H2-PD group as compared to the control and PD groups. Also, results of immunostaining analysis revealed increased vimentin and apoptotic cells in the membrane of the PD group, indicating that H2 may play a role in ameliorating PDS-induced peritoneal injury and preserving peritoneal integrity. In the clinical trial with 6 prevalent PD patients, all subjects completed the study with no adverse effects. Moreover, there were substantial changes in surrogate markers, such as increased CA125 and mesothelin, in the effluent in selected cases, suggesting enhanced mesothelial regeneration by H2.

Original languageEnglish
Pages (from-to)162-170
Number of pages9
JournalContributions to nephrology
Volume196
DOIs
Publication statusPublished - 2018

ASJC Scopus subject areas

  • Nephrology

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