Translational inhibition by heme of the synthesis of hepatic δ-aminolevulinate synthase in a cell-free system

Masayuki Yamamoto, Norio Hayashi, Goro Kikuchi

Research output: Contribution to journalArticlepeer-review

33 Citations (Scopus)

Abstract

Synthesis of δ-aminolevulinate synthase in a rabbit reticulocyte lysate system directed by total polysomes from the liver of allylisopropylacetamide-treated rats was studied with the combined use of [3H]leucine and a specific rabbit antibody. The protein synthesis observed in the cell-free system employed represented mainly the peptide chain elongation and its termination rather than the net synthesis involving initiation. Synthesis of δ-aminolevulinate synthase in this cell-free system was inhibited progressively with the increased addition of hemin; the synthesis was reduced to about 40% by about 30 μM hemin. Synthesis of total protein, however, was not significantly affected by the addition of hemin. The data obtained suggest that heme inhibits a peptide chain elongation step in the synthesis of δ-aminolevulinate synthase.

Original languageEnglish
Pages (from-to)225-231
Number of pages7
JournalBiochemical and biophysical research communications
Volume115
Issue number1
DOIs
Publication statusPublished - 1983 Aug 30

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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