Translational control of maternal glp-1 mRNA by POS-1 and its interacting protein SPN-4 in Caenorhabditis elegans

Ken Ichi Ogura, Norihito Kishimoto, Shohei Mitani, Keiko Gengyo-Ando, Yuji Kohara

Research output: Contribution to journalReview articlepeer-review

70 Citations (Scopus)

Abstract

The translation of maternal glp-1 mRNAs is regulated temporally and spatially in C. elegans embryos. The 3′ UTR (untranslated region) of the maternal glp-1 mRNA is important for both kinds of regulation. The spatial control region is required to suppress translation in the posterior blastomeres. The temporal one is required to suppress translation in oocytes and one-cell stage embryos. We show that a CCCH zinc-finger protein, POS-1, represses glp-1 mRNA translation by binding to the spatial control region. We identified an RNP-type RNA-binding protein, SPN-4, as a POS-1-interacting protein. SPN-4 is present developmentally from the oocyte to the early embryo and its distribution overlaps with that of POS-1 in the cytoplasm and P granules of the posterior blastomeres. SPN-4 binds to a subregion of the temporal control region in the 3′ UTR and is required for the translation of glp-1 mRNA in the anterior blastomeres. We propose that the balance between POS-1 and SPN-4 controls the translation of maternal glp-1 mRNA.

Original languageEnglish
Pages (from-to)2495-2503
Number of pages9
JournalDevelopment
Volume130
Issue number11
DOIs
Publication statusPublished - 2003 Jun
Externally publishedYes

Keywords

  • 3′ UTR
  • Caenorhabditis elegans
  • Maternal mRNA
  • RNA-binding protein
  • Translational control

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology

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