Translation of the poly(A) tail plays crucial roles in nonstop mRNA surveillance via translation repression and protein destabilization by proteasome in yeast

Sayoko Ito-Harashima, Kazushige Kuroha, Tsuyako Tatematsu, Toshifumi Inada

Research output: Contribution to journalArticlepeer-review

135 Citations (Scopus)

Abstract

mRNA surveillance system represses the expression of nonstop mRNA by rapid mRNA degradation and translation repression. Here we show that the level of protein product of nonstop mRNA containing a poly(A) tail was reduced 100-fold, and this reduction was due to rapid mRNA degradation, translation repression, and protein destabilization, at least in part, by the proteasome. Insertion of a poly(A) tract upstream of a termination codon resulted in translation repression and protein destabilization, but not rapid mRNA decay. We propose that translation of the poly(A) tail plays crucial roles in nonstop mRNA surveillance via translation repression and protein destabilization.

Original languageEnglish
Pages (from-to)519-524
Number of pages6
JournalGenes and Development
Volume21
Issue number5
DOIs
Publication statusPublished - 2007 Mar 1

Keywords

  • Nonstop mRNA
  • Poly(A)
  • Polylysine
  • Proteasome
  • Translational repression

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

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