Translation arrest as a protein quality control system for aberrant translation of the 3′-UTR in mammalian cells

Satoshi Hashimoto, Risa Nobuta, Toshiaki Izawa, Toshifumi Inada

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

Read-through or mutations of a stop codon resulting in translation of the 3′-UTR produce potentially toxic C-terminally extended proteins. However, quality control mechanisms for such proteins are poorly understood in mammalian cells. Here, a comprehensive analysis of the 3′-UTRs of genes associated with hereditary diseases identified novel arrest-inducing sequences in the 3′-UTRs of 23 genes that can repress the levels of their protein products. In silico analysis revealed that the hydrophobicity of the polypeptides encoded in the 3′-UTRs is correlated with arrest efficiency. These results provide new insight into quality control mechanisms mediated by 3′-UTRs to prevent the production of C-terminally extended cytotoxic proteins.

Original languageEnglish
Pages (from-to)777-787
Number of pages11
JournalFEBS Letters
Volume593
Issue number8
DOIs
Publication statusPublished - 2019 Apr

Keywords

  • 3′-UTR
  • hydrophobicity
  • translation arrest

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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