Transitin, a nestin-like intermediate filament protein, mediates cortical localization and the lateral transport of Numb in mitotic avian neuroepithelial cells

Yoshio Wakamatsu, Noriko Nakamura, Ju Ahng Lee, Gregory J. Cole, Noriko Osumi

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Neuroepithelium is an apicobasally polarized tissue that contains neural stem cells and gives rise to neurons and glial cells of the central nervous system. The cleavage orientation of neural stem cells is thought to be important for asymmetric segregation of fate-determinants, such as Numb. Here, we show that an intermediate filament protein, transitin, colocalizes with Numb in the cell cortex of mitotic neuroepithelial cells, and that transitin anchors Numb via a physical interaction. Detailed immunohistological and time-lapse analyses reveal that basal Numb-transitin complexes shift laterally during mitosis, allowing asymmetric segregation of Numb-transitin to one of the daughter cells, even when the cell cleavage plane is perpendicular to the ventricular surface. In addition, RNA interference (RNAi) knockdown of the transitin gene reveals its involvement in neurogenesis. These results indicate that transitin has important roles in determining the intracellular localization of Numb, which regulates neurogenesis in the developing nervous system of avian embryos.

Original languageEnglish
Pages (from-to)2425-2433
Number of pages9
JournalDevelopment
Volume134
Issue number13
DOIs
Publication statusPublished - 2007 Jul 1

Keywords

  • Asymmetric cell division
  • Chick
  • Nestin
  • Neuroepithelium
  • Numb
  • Transitin

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology

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