Transient expression of an IL-23R extracellular domain Fc fusion protein in CHO vs. HEK cells results in improved plasma exposure

Ka Fai Suen, Mary S. Turner, Feng Gao, Bo Liu, Alana Althage, Anthony Slavin, Weijia Ou, Elizabeth Zuo, Michael Eckart, Tomohisa Ogawa, Masao Yamada, Tove Tuntland, Jennifer L. Harris, John W. Trauger

Research output: Contribution to journalArticlepeer-review

24 Citations (Scopus)

Abstract

Transient transfection of mammalian cells in suspension culture has recently emerged as a very useful method for production of research-scale quantities of recombinant proteins. The most commonly used cell lines for this purpose are suspension-adapted HEK and CHO cells. We report here that the plasma exposure in mice of an IL-23R extracellular domain Fc fusion protein (IL23R-Fc) differed dramatically depending on whether the protein was prepared by transient transfection of HEK or CHO cells. Specifically, IL23R-Fc expressed using CHO cells had about 30-fold higher in vivo plasma exposure compared to the HEK-expressed protein. In contrast to their differing plasma exposures, the HEK- and CHO-expressed proteins had equivalent in vitro biological activity. Characterization of the CHO- and HEK-expressed IL23R-Fc proteins indicated that the differences in in vivo plasma exposure between them are due to differential glycosylation.

Original languageEnglish
Pages (from-to)96-102
Number of pages7
JournalProtein Expression and Purification
Volume71
Issue number1
DOIs
Publication statusPublished - 2010 May
Externally publishedYes

Keywords

  • CHO cells
  • Glycosylation
  • HEK cells
  • Pharmacokinetics
  • Recombinant protein expression
  • Systemic exposure
  • Transient transfection

ASJC Scopus subject areas

  • Biotechnology

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