Transient expression of an IL-23R extracellular domain Fc fusion protein in CHO vs. HEK cells results in improved plasma exposure

Ka Fai Suen; Mary S. Turner; Feng Gao; Bo Liu; Alana Althage; Anthony Slavin; Weijia Ou; Elizabeth Zuo; Michael Eckart; Tomohisa Ogawa; Masao Yamada; Tove Tuntland; Jennifer L. Harris; John W. Trauger

doi:10.1016/j.pep.2009.12.015

Transient expression of an IL-23R extracellular domain Fc fusion protein in CHO vs. HEK cells results in improved plasma exposure

Ka Fai Suen, Mary S. Turner, Feng Gao, Bo Liu, Alana Althage, Anthony Slavin, Weijia Ou, Elizabeth Zuo, Michael Eckart, Tomohisa Ogawa, Masao Yamada, Tove Tuntland, Jennifer L. Harris, John W. Trauger

Research output: Contribution to journal › Article › peer-review

28 Citations (Scopus)

Abstract

Transient transfection of mammalian cells in suspension culture has recently emerged as a very useful method for production of research-scale quantities of recombinant proteins. The most commonly used cell lines for this purpose are suspension-adapted HEK and CHO cells. We report here that the plasma exposure in mice of an IL-23R extracellular domain Fc fusion protein (IL23R-Fc) differed dramatically depending on whether the protein was prepared by transient transfection of HEK or CHO cells. Specifically, IL23R-Fc expressed using CHO cells had about 30-fold higher in vivo plasma exposure compared to the HEK-expressed protein. In contrast to their differing plasma exposures, the HEK- and CHO-expressed proteins had equivalent in vitro biological activity. Characterization of the CHO- and HEK-expressed IL23R-Fc proteins indicated that the differences in in vivo plasma exposure between them are due to differential glycosylation.

Original language	English
Pages (from-to)	96-102
Number of pages	7
Journal	Protein Expression and Purification
Volume	71
Issue number	1
DOIs	https://doi.org/10.1016/j.pep.2009.12.015
Publication status	Published - 2010 May
Externally published	Yes

Keywords

CHO cells
Glycosylation
HEK cells
Pharmacokinetics
Recombinant protein expression
Systemic exposure
Transient transfection

ASJC Scopus subject areas

Biotechnology

Access to Document

10.1016/j.pep.2009.12.015

Cite this

Suen, K. F., Turner, M. S., Gao, F., Liu, B., Althage, A., Slavin, A., Ou, W., Zuo, E., Eckart, M., Ogawa, T., Yamada, M., Tuntland, T., Harris, J. L., & Trauger, J. W. (2010). Transient expression of an IL-23R extracellular domain Fc fusion protein in CHO vs. HEK cells results in improved plasma exposure. Protein Expression and Purification, 71(1), 96-102. https://doi.org/10.1016/j.pep.2009.12.015

Suen, KF, Turner, MS, Gao, F, Liu, B, Althage, A, Slavin, A, Ou, W, Zuo, E, Eckart, M, Ogawa, T, Yamada, M, Tuntland, T, Harris, JL & Trauger, JW 2010, 'Transient expression of an IL-23R extracellular domain Fc fusion protein in CHO vs. HEK cells results in improved plasma exposure', Protein Expression and Purification, vol. 71, no. 1, pp. 96-102. https://doi.org/10.1016/j.pep.2009.12.015

@article{fcb34def81d24de8bc540030426c3855,

title = "Transient expression of an IL-23R extracellular domain Fc fusion protein in CHO vs. HEK cells results in improved plasma exposure",

abstract = "Transient transfection of mammalian cells in suspension culture has recently emerged as a very useful method for production of research-scale quantities of recombinant proteins. The most commonly used cell lines for this purpose are suspension-adapted HEK and CHO cells. We report here that the plasma exposure in mice of an IL-23R extracellular domain Fc fusion protein (IL23R-Fc) differed dramatically depending on whether the protein was prepared by transient transfection of HEK or CHO cells. Specifically, IL23R-Fc expressed using CHO cells had about 30-fold higher in vivo plasma exposure compared to the HEK-expressed protein. In contrast to their differing plasma exposures, the HEK- and CHO-expressed proteins had equivalent in vitro biological activity. Characterization of the CHO- and HEK-expressed IL23R-Fc proteins indicated that the differences in in vivo plasma exposure between them are due to differential glycosylation.",

keywords = "CHO cells, Glycosylation, HEK cells, Pharmacokinetics, Recombinant protein expression, Systemic exposure, Transient transfection",

author = "Suen, {Ka Fai} and Turner, {Mary S.} and Feng Gao and Bo Liu and Alana Althage and Anthony Slavin and Weijia Ou and Elizabeth Zuo and Michael Eckart and Tomohisa Ogawa and Masao Yamada and Tove Tuntland and Harris, {Jennifer L.} and Trauger, {John W.}",

year = "2010",

month = may,

doi = "10.1016/j.pep.2009.12.015",

language = "English",

volume = "71",

pages = "96--102",

journal = "Protein Expression and Purification",

issn = "1046-5928",

publisher = "Academic Press Inc.",

number = "1",

}

TY - JOUR

T1 - Transient expression of an IL-23R extracellular domain Fc fusion protein in CHO vs. HEK cells results in improved plasma exposure

AU - Suen, Ka Fai

AU - Turner, Mary S.

AU - Gao, Feng

AU - Liu, Bo

AU - Althage, Alana

AU - Slavin, Anthony

AU - Ou, Weijia

AU - Zuo, Elizabeth

AU - Eckart, Michael

AU - Ogawa, Tomohisa

AU - Yamada, Masao

AU - Tuntland, Tove

AU - Harris, Jennifer L.

AU - Trauger, John W.

PY - 2010/5

Y1 - 2010/5

N2 - Transient transfection of mammalian cells in suspension culture has recently emerged as a very useful method for production of research-scale quantities of recombinant proteins. The most commonly used cell lines for this purpose are suspension-adapted HEK and CHO cells. We report here that the plasma exposure in mice of an IL-23R extracellular domain Fc fusion protein (IL23R-Fc) differed dramatically depending on whether the protein was prepared by transient transfection of HEK or CHO cells. Specifically, IL23R-Fc expressed using CHO cells had about 30-fold higher in vivo plasma exposure compared to the HEK-expressed protein. In contrast to their differing plasma exposures, the HEK- and CHO-expressed proteins had equivalent in vitro biological activity. Characterization of the CHO- and HEK-expressed IL23R-Fc proteins indicated that the differences in in vivo plasma exposure between them are due to differential glycosylation.

AB - Transient transfection of mammalian cells in suspension culture has recently emerged as a very useful method for production of research-scale quantities of recombinant proteins. The most commonly used cell lines for this purpose are suspension-adapted HEK and CHO cells. We report here that the plasma exposure in mice of an IL-23R extracellular domain Fc fusion protein (IL23R-Fc) differed dramatically depending on whether the protein was prepared by transient transfection of HEK or CHO cells. Specifically, IL23R-Fc expressed using CHO cells had about 30-fold higher in vivo plasma exposure compared to the HEK-expressed protein. In contrast to their differing plasma exposures, the HEK- and CHO-expressed proteins had equivalent in vitro biological activity. Characterization of the CHO- and HEK-expressed IL23R-Fc proteins indicated that the differences in in vivo plasma exposure between them are due to differential glycosylation.

KW - CHO cells

KW - Glycosylation

KW - HEK cells

KW - Pharmacokinetics

KW - Recombinant protein expression

KW - Systemic exposure

KW - Transient transfection

UR - http://www.scopus.com/inward/record.url?scp=77049087515&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77049087515&partnerID=8YFLogxK

U2 - 10.1016/j.pep.2009.12.015

DO - 10.1016/j.pep.2009.12.015

M3 - Article

C2 - 20045465

AN - SCOPUS:77049087515

VL - 71

SP - 96

EP - 102

JO - Protein Expression and Purification

JF - Protein Expression and Purification

SN - 1046-5928

IS - 1

ER -

Transient expression of an IL-23R extracellular domain Fc fusion protein in CHO vs. HEK cells results in improved plasma exposure

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this