Abstract
Transient transfection of mammalian cells in suspension culture has recently emerged as a very useful method for production of research-scale quantities of recombinant proteins. The most commonly used cell lines for this purpose are suspension-adapted HEK and CHO cells. We report here that the plasma exposure in mice of an IL-23R extracellular domain Fc fusion protein (IL23R-Fc) differed dramatically depending on whether the protein was prepared by transient transfection of HEK or CHO cells. Specifically, IL23R-Fc expressed using CHO cells had about 30-fold higher in vivo plasma exposure compared to the HEK-expressed protein. In contrast to their differing plasma exposures, the HEK- and CHO-expressed proteins had equivalent in vitro biological activity. Characterization of the CHO- and HEK-expressed IL23R-Fc proteins indicated that the differences in in vivo plasma exposure between them are due to differential glycosylation.
Original language | English |
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Pages (from-to) | 96-102 |
Number of pages | 7 |
Journal | Protein Expression and Purification |
Volume | 71 |
Issue number | 1 |
DOIs | |
Publication status | Published - 2010 May |
Externally published | Yes |
Keywords
- CHO cells
- Glycosylation
- HEK cells
- Pharmacokinetics
- Recombinant protein expression
- Systemic exposure
- Transient transfection
ASJC Scopus subject areas
- Biotechnology