Transgenic plant production mediated by Agrobacterium in Indica rice

Hamid Rashid, Shuuji Yokoi, Kinya Toriyama, Kokichi Hinata

Research output: Contribution to journalArticle

241 Citations (Scopus)

Abstract

A reproducible system has been developed for the production of transgenic plants in indica rice using Agrobacterium-mediated gene transfer. Three-week-old scutella calli served as an excellent starting material. These were infected with an Agrobacterium tumefaciens strain EHA101 carrying a plasmid pIG121Hm containing genes for β-glucuronidase (GUS) and hygromycin resistnace (HygR). Hygromycin (50 mg/l) was used as a selectable agent. Inclusion of acetosyringone (50μM) in the Agrobacterium suspension and co-culture media proved to be indispensable for successful transformation. Transformation efficiency of Basmati 370 was 22% which was as high as reported in japonica rice and dicots. A large number of morphologically normal, fertile transgenic plants were obtained. Integration of foreign genes into the genome of transgenic plants was confirmed by Southern blot analysis. GUS and HygR genes were inherited and expressed in R1 progeny. Mendelian segregation was observed in some R1 progeny.

Original languageEnglish
Pages (from-to)727-730
Number of pages4
JournalPlant Cell Reports
Volume15
Issue number10
DOIs
Publication statusPublished - 1996 Jun 1

Keywords

  • Acetosyringone
  • Agrobacterium
  • Indica rice
  • Oryza sativa L.
  • Transgenic plants

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

Fingerprint Dive into the research topics of 'Transgenic plant production mediated by Agrobacterium in Indica rice'. Together they form a unique fingerprint.

  • Cite this