Abstract
A high frequency shoot regeneration (80%) was developed from callus of leaf discs and stem internodes of Moricandia arvensis. Leaf discs were shown to be a preferable starting material for transformation experiments. Agrobacterium tumefaciens strain GV3101/pMP90 used in this study contained a binary vector with genes for kanamycin resistance, hygromycin resistance and β-glucuronidase (GUS). Maximum transformation efficiency (10.3%) was achieved by using kanamycin at the rate of 200 mg/l as a selection agent. Presence of tobacco suspension culture during co-cultivation and a pre- selection period of seven days after co-cultivation was essential for successful transformation. Transgenic plants grew to maturity and exhibited flowering in a glasshouse. GUS activity was evident in all parts of leaf and the presence of GUS gene in plant gemone was confirmed by PCR analysis.
Original language | English |
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Pages (from-to) | 799-803 |
Number of pages | 5 |
Journal | Plant Cell Reports |
Volume | 15 |
Issue number | 11 |
DOIs | |
Publication status | Published - 1996 |
Keywords
- Agrobacterium tumefaciens
- Leaf disc transformation
- Moricandia arvensis
- Transgenic plants
ASJC Scopus subject areas
- Agronomy and Crop Science
- Plant Science