Transgenic plant production from leaf discs of Moricandia arvensis using Agrobacterium tumefaciens

Hamid Rashid; Kinya Toriyama; Kokichi Hinata

doi:10.1007/BF00233143

Transgenic plant production from leaf discs of Moricandia arvensis using Agrobacterium tumefaciens

Hamid Rashid, Kinya Toriyama, Kokichi Hinata

AGR - Agricultural Bioscience

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

A high frequency shoot regeneration (80%) was developed from callus of leaf discs and stem internodes of Moricandia arvensis. Leaf discs were shown to be a preferable starting material for transformation experiments. Agrobacterium tumefaciens strain GV3101/pMP90 used in this study contained a binary vector with genes for kanamycin resistance, hygromycin resistance and β-glucuronidase (GUS). Maximum transformation efficiency (10.3%) was achieved by using kanamycin at the rate of 200 mg/l as a selection agent. Presence of tobacco suspension culture during co-cultivation and a pre- selection period of seven days after co-cultivation was essential for successful transformation. Transgenic plants grew to maturity and exhibited flowering in a glasshouse. GUS activity was evident in all parts of leaf and the presence of GUS gene in plant gemone was confirmed by PCR analysis.

Original language	English
Pages (from-to)	799-803
Number of pages	5
Journal	Plant Cell Reports
Volume	15
Issue number	11
DOIs	https://doi.org/10.1007/BF00233143
Publication status	Published - 1996

Keywords

Agrobacterium tumefaciens
Leaf disc transformation
Moricandia arvensis
Transgenic plants

ASJC Scopus subject areas

Agronomy and Crop Science
Plant Science

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10.1007/BF00233143

Cite this

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title = "Transgenic plant production from leaf discs of Moricandia arvensis using Agrobacterium tumefaciens",

abstract = "A high frequency shoot regeneration (80%) was developed from callus of leaf discs and stem internodes of Moricandia arvensis. Leaf discs were shown to be a preferable starting material for transformation experiments. Agrobacterium tumefaciens strain GV3101/pMP90 used in this study contained a binary vector with genes for kanamycin resistance, hygromycin resistance and β-glucuronidase (GUS). Maximum transformation efficiency (10.3%) was achieved by using kanamycin at the rate of 200 mg/l as a selection agent. Presence of tobacco suspension culture during co-cultivation and a pre- selection period of seven days after co-cultivation was essential for successful transformation. Transgenic plants grew to maturity and exhibited flowering in a glasshouse. GUS activity was evident in all parts of leaf and the presence of GUS gene in plant gemone was confirmed by PCR analysis.",

keywords = "Agrobacterium tumefaciens, Leaf disc transformation, Moricandia arvensis, Transgenic plants",

author = "Hamid Rashid and Kinya Toriyama and Kokichi Hinata",

year = "1996",

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journal = "Plant Cell Reports",

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T1 - Transgenic plant production from leaf discs of Moricandia arvensis using Agrobacterium tumefaciens

AU - Rashid, Hamid

AU - Toriyama, Kinya

AU - Hinata, Kokichi

PY - 1996

Y1 - 1996

N2 - A high frequency shoot regeneration (80%) was developed from callus of leaf discs and stem internodes of Moricandia arvensis. Leaf discs were shown to be a preferable starting material for transformation experiments. Agrobacterium tumefaciens strain GV3101/pMP90 used in this study contained a binary vector with genes for kanamycin resistance, hygromycin resistance and β-glucuronidase (GUS). Maximum transformation efficiency (10.3%) was achieved by using kanamycin at the rate of 200 mg/l as a selection agent. Presence of tobacco suspension culture during co-cultivation and a pre- selection period of seven days after co-cultivation was essential for successful transformation. Transgenic plants grew to maturity and exhibited flowering in a glasshouse. GUS activity was evident in all parts of leaf and the presence of GUS gene in plant gemone was confirmed by PCR analysis.

AB - A high frequency shoot regeneration (80%) was developed from callus of leaf discs and stem internodes of Moricandia arvensis. Leaf discs were shown to be a preferable starting material for transformation experiments. Agrobacterium tumefaciens strain GV3101/pMP90 used in this study contained a binary vector with genes for kanamycin resistance, hygromycin resistance and β-glucuronidase (GUS). Maximum transformation efficiency (10.3%) was achieved by using kanamycin at the rate of 200 mg/l as a selection agent. Presence of tobacco suspension culture during co-cultivation and a pre- selection period of seven days after co-cultivation was essential for successful transformation. Transgenic plants grew to maturity and exhibited flowering in a glasshouse. GUS activity was evident in all parts of leaf and the presence of GUS gene in plant gemone was confirmed by PCR analysis.

KW - Agrobacterium tumefaciens

KW - Leaf disc transformation

KW - Moricandia arvensis

KW - Transgenic plants

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ER -

Transgenic plant production from leaf discs of Moricandia arvensis using Agrobacterium tumefaciens

Abstract

Keywords

ASJC Scopus subject areas

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