Transgenic plant production from leaf discs of Moricandia arvensis using Agrobacterium tumefaciens

Hamid Rashid, Kinya Toriyama, Kokichi Hinata

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

A high frequency shoot regeneration (80%) was developed from callus of leaf discs and stem internodes of Moricandia arvensis. Leaf discs were shown to be a preferable starting material for transformation experiments. Agrobacterium tumefaciens strain GV3101/pMP90 used in this study contained a binary vector with genes for kanamycin resistance, hygromycin resistance and β-glucuronidase (GUS). Maximum transformation efficiency (10.3%) was achieved by using kanamycin at the rate of 200 mg/l as a selection agent. Presence of tobacco suspension culture during co-cultivation and a pre- selection period of seven days after co-cultivation was essential for successful transformation. Transgenic plants grew to maturity and exhibited flowering in a glasshouse. GUS activity was evident in all parts of leaf and the presence of GUS gene in plant gemone was confirmed by PCR analysis.

Original languageEnglish
Pages (from-to)799-803
Number of pages5
JournalPlant Cell Reports
Volume15
Issue number11
DOIs
Publication statusPublished - 1996 Jan 1

Keywords

  • Agrobacterium tumefaciens
  • Leaf disc transformation
  • Moricandia arvensis
  • Transgenic plants

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

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