TY - JOUR
T1 - Transgenic overexpression of Reg protein caused gastric cell proliferation and differentiation along parietal cell and chief cell lineages
AU - Miyaoka, Youichi
AU - Kadowaki, Yasunori
AU - Ishihara, Shunji
AU - Ose, Takayuki
AU - Fukuhara, Hiroyuki
AU - Kazumori, Hideaki
AU - Takasawa, Shin
AU - Okamoto, Hiroshi
AU - Chiba, Tsutomu
AU - Kinoshita, Yoshikazu
PY - 2004/4/29
Y1 - 2004/4/29
N2 - Reg (regenerating gene product) was originally identified as a growth factor involved in pancreatic regeneration. During the healing course of gastric erosion, Reg expression is highly increased in the enterochromaffin-like (ECL) cells surrounding the ulcer crater, suggesting its role as a regulator of gastric mucosal regeneration. However, there has been no direct in vivo evidence of a growth-promoting role of Reg for the gastric mucosal cells. In the current study, Reg-transgenic mice were created and gastric mucosa were analysed for histological changes. Transgenic mice showed a marked increase in the thickness of the fundic mucosa. Anti-proliferating cell nuclear antigen (PCNA) staining of the fundic mucosa demonstrated the enlargement of the proliferating neck zone and the lower PCNA-negative zone. Histological analysis employing antibodies against cell-type markers revealed expansion of the chief cell and parietal cell populations and no change in the number of surface mucus-producing cells, ECL cells, or G cells. In conclusion, Reg has a growth-promoting effect on gastric progenitor cells and an activity to direct the differentiation of the cells into chief cell and parietal cell lineages. This was in contrast to other factors, all of which had been shown to drive differentiation towards mucus producing cells in vivo. In the injured gastric mucosa, Reg may play a unique and important part in the reconstruction of the properly organized mucosal architecture.
AB - Reg (regenerating gene product) was originally identified as a growth factor involved in pancreatic regeneration. During the healing course of gastric erosion, Reg expression is highly increased in the enterochromaffin-like (ECL) cells surrounding the ulcer crater, suggesting its role as a regulator of gastric mucosal regeneration. However, there has been no direct in vivo evidence of a growth-promoting role of Reg for the gastric mucosal cells. In the current study, Reg-transgenic mice were created and gastric mucosa were analysed for histological changes. Transgenic mice showed a marked increase in the thickness of the fundic mucosa. Anti-proliferating cell nuclear antigen (PCNA) staining of the fundic mucosa demonstrated the enlargement of the proliferating neck zone and the lower PCNA-negative zone. Histological analysis employing antibodies against cell-type markers revealed expansion of the chief cell and parietal cell populations and no change in the number of surface mucus-producing cells, ECL cells, or G cells. In conclusion, Reg has a growth-promoting effect on gastric progenitor cells and an activity to direct the differentiation of the cells into chief cell and parietal cell lineages. This was in contrast to other factors, all of which had been shown to drive differentiation towards mucus producing cells in vivo. In the injured gastric mucosa, Reg may play a unique and important part in the reconstruction of the properly organized mucosal architecture.
KW - Gastric cells
KW - Growth factor
KW - Reg
KW - Transgenic mice
UR - http://www.scopus.com/inward/record.url?scp=2442709188&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=2442709188&partnerID=8YFLogxK
U2 - 10.1038/sj.onc.1207333
DO - 10.1038/sj.onc.1207333
M3 - Article
C2 - 15116092
AN - SCOPUS:2442709188
VL - 23
SP - 3572
EP - 3579
JO - Oncogene
JF - Oncogene
SN - 0950-9232
IS - 20
ER -