Transgenic mouse lines expressing synaptopHluorin in hippocampus and cerebellar cortex

Rikita Araki, Hiroyuki Sakagami, Yuchio Yanagawa, Takuya Hikima, Toru Ishizuka, Hiromu Yawo

    Research output: Contribution to journalArticlepeer-review

    14 Citations (Scopus)

    Abstract

    We generated six transgenic mouse lines in which synaptopHluorin (SpH), one of green fluorescent protein-based sensors of vesicular exocytosis, was expressed under the control of neuron-specific Thy-1.2 promoter. In situ hybridization study revealed that SpH mRNA was expressed in a broad spectrum of brain regions in four of them, whereas in others it was expressed in the specific regions of the hippocampus. In one particular line, SpH immunoreactivity was specifically observed in the mossy fiber presynaptic terminals of both hippocampus and cerebellar cortex, The fluorescence intensity of these presynaptic terminals was somewhat decreased by acidic buffer superfusion and greatly increased by vesicular neutralization of pH, indicating that the SpH molecules are mainly distributed in the synaptic vesicles. The exocytosis-dependent fluorescence increment was measured upon activation of a single presynaptic terminal. These transgenic lines are expected to facilitate morphological and physiological studies of presynaptic terminals in a variety of regions of the brain.

    Original languageEnglish
    Pages (from-to)53-60
    Number of pages8
    JournalGenesis
    Volume42
    Issue number2
    DOIs
    Publication statusPublished - 2005 Jun

    Keywords

    • Brain
    • Exocytosis
    • GFP
    • Synapse
    • Thy-1 promoter
    • VAMP

    ASJC Scopus subject areas

    • Genetics
    • Endocrinology
    • Cell Biology

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