TY - JOUR
T1 - Transgenic mice overexpressing human vasoactive intestinal peptide (VIP) gene in pancreatic β cells. Evidence for improved glucose tolerance and enhanced insulin secretion by VIP and PHM-27 in vivo
AU - Kato, I.
AU - Suzuki, Y.
AU - Akabane, A.
AU - Yonekura, H.
AU - Tanaka, O.
AU - Kondo, H.
AU - Takasawa, S.
AU - Yoshimoto, T.
AU - Okamoto, H.
N1 - Copyright:
Copyright 2004 Elsevier B.V., All rights reserved.
PY - 1994
Y1 - 1994
N2 - Vasoactive intestinal peptide (VIP), a 28-amino acid peptide hormone, plays many physiological roles in the peripheral and central nervous systems. It has been proposed that endogenous VIP released from VIP-containing nerves is involved in the regulation of the secretory function of the endocrine pancreas. To test this hypothesis in vivo, we produced transgenic mice carrying the human VIP/peptide histidine methionine 27 (PHM-27) gene under the control of insulin promoter. In immunohistochemical analyses of islets, all the islet β cells of transgenic mice were intensely stained for both VIP and PHM-27, consistent with the fact that these two peptides are encoded in a single mRNA (Itoh, N., Obata, K., Yanaihara, N., and Okamoto, H. (1983) Nature 304, 547-549). VIP was efficiently secreted from isolated transgenic islets in vitro. The blood glucose assays in free-fed mice indicated that the transgene lowered the blood glucose levels of transgenic mice (128 ± 4 mg/dl) by about 20% below control levels (155 ± 6 mg/dl). In the glucose tolerance test, at 60 min after glucose administration, the transgenic blood glucose levels (129 ± 12 mg/dl) were much lower than control levels (175 ± 13 mg/dl). The transgenic serum insulin levels at 15 min after glucose administration were 2.5-3.0-fold higher than control levels. The transgene was also effective in ameliorating glucose intolerance of 70% depancreatized mice. These results indicate that VIP and PHM-27 produced from the transgenic β cells efficiently enhance glucose-induced insulin secretion from β cells by an autocrine mechanism. These results also suggest that genetic manipulation of islet β cells by the human VIP/PHM-27 gene or delivery of VIP to β cells may ultimately provide a valuable approach to enhancing insulin secretion in clinical diabetes.
AB - Vasoactive intestinal peptide (VIP), a 28-amino acid peptide hormone, plays many physiological roles in the peripheral and central nervous systems. It has been proposed that endogenous VIP released from VIP-containing nerves is involved in the regulation of the secretory function of the endocrine pancreas. To test this hypothesis in vivo, we produced transgenic mice carrying the human VIP/peptide histidine methionine 27 (PHM-27) gene under the control of insulin promoter. In immunohistochemical analyses of islets, all the islet β cells of transgenic mice were intensely stained for both VIP and PHM-27, consistent with the fact that these two peptides are encoded in a single mRNA (Itoh, N., Obata, K., Yanaihara, N., and Okamoto, H. (1983) Nature 304, 547-549). VIP was efficiently secreted from isolated transgenic islets in vitro. The blood glucose assays in free-fed mice indicated that the transgene lowered the blood glucose levels of transgenic mice (128 ± 4 mg/dl) by about 20% below control levels (155 ± 6 mg/dl). In the glucose tolerance test, at 60 min after glucose administration, the transgenic blood glucose levels (129 ± 12 mg/dl) were much lower than control levels (175 ± 13 mg/dl). The transgenic serum insulin levels at 15 min after glucose administration were 2.5-3.0-fold higher than control levels. The transgene was also effective in ameliorating glucose intolerance of 70% depancreatized mice. These results indicate that VIP and PHM-27 produced from the transgenic β cells efficiently enhance glucose-induced insulin secretion from β cells by an autocrine mechanism. These results also suggest that genetic manipulation of islet β cells by the human VIP/PHM-27 gene or delivery of VIP to β cells may ultimately provide a valuable approach to enhancing insulin secretion in clinical diabetes.
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M3 - Article
C2 - 8063743
AN - SCOPUS:0027994096
VL - 269
SP - 21223
EP - 21228
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
SN - 0021-9258
IS - 33
ER -