Transgenic expression of Telomerase reverse transcriptase (Tert) improves cell proliferation of primary cells and enhances reprogramming efficiency into the induced pluripotent stem cell

Shizu Hidema, Tomokazu Fukuda, Shiori Date, Yuko Tokitake, Yasuhisa Matsui, Hiroki Sasaki, Katsuhiko Nishimori Nishimori

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The enzymatic activity of telomerase is important for the extension of the telomere repeat sequence and overcoming cellular senescence. We generated a conditional transgenic mouse line, carrying the telomerase reverse transcriptase (Tert) expression cassette, controlled by the Cre-loxP-mediated recombination. In our study, Cre recombinase expression efficiently activated Tert expression, resulting in its increased enzymatic activity, which extended the period of cellular proliferation until the keratinocytes entered senescence. This suggests that transgenic Tert expression is effective in enhancing primary cell proliferation. Notably, Tert expression increased colony formation of induced pluripotent stem (iPS) cells after the introduction of four reprogramming factors, Oct-4, klf4, SOX-2, and c-Myc into the transgenic fibroblasts. To the best of our knowledge, this is the first study to show that the transgenic Tert expression enhances reprogramming efficiency of iPS cells, which indicates a critical role for Tert in the reprogramming process.

Original languageEnglish
Pages (from-to)1925-1933
Number of pages9
JournalBioscience, Biotechnology and Biochemistry
Volume80
Issue number10
DOIs
Publication statusPublished - 2016 Jan 1

Keywords

  • Keratinocyte
  • Primary cell
  • Proliferation
  • Reprogramming
  • Telomerase

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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