Transgenesis by microparticle bombardment for live imaging of fluorescent proteins in Pristionchus pacificus germline and early embryos

Satoshi Namai, Asako Sugimoto

    Research output: Contribution to journalArticle

    5 Citations (Scopus)

    Abstract

    Pristionchus pacificus is a free-living nematode used as a model organism for evolutionary developmental and ecological biology. Although a transgenic technique to form complex arrays by microinjection has been established in P. pacificus, transgene expression from the array in the germline and early embryos tends to be silenced. Here, we established a method to integrate transgenes into the genome of P. pacificus using microparticle bombardment with hygromycin B selection. Additionally, we isolated a mutant exhibiting significantly lower autofluorescence in the germline and early embryos, facilitating visualization of transgene-derived fluorescent proteins for live imaging. Transgenic lines constructed using these tools successfully expressed GFP-tagged proteins in the germline and early embryos and enabled live imaging of chromosomes, microtubules, and centrosomes.

    Original languageEnglish
    Pages (from-to)75-82
    Number of pages8
    JournalDevelopment Genes and Evolution
    Volume228
    Issue number1
    DOIs
    Publication statusPublished - 2018 Jan 1

    Keywords

    • Microparticle bombardment
    • Pristionchus pacificus
    • Transgenesis

    ASJC Scopus subject areas

    • Genetics
    • Developmental Biology

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