Transformation System for Aspergillus oryzae with Double Auxotrophic Mutations, niaD and sC

Osamu Yamada, Byung Rho Lee, Katsuya Gomi

Research output: Contribution to journalArticlepeer-review

103 Citations (Scopus)

Abstract

We developed a transformation system for Aspergillus oryzae using the Aspergillus nidulans sC gene encoding ATP sulfurylase as a selectable marker. The sC mutants can be readily isolated by positive selection for selenate resistance, thereby the niaD mutant strain of A. oryzae was bestowed with the sC mutation. Transformation of the A. oryzae host (niaD, sC) with the plasmid carrying A. nidulans sC gave random and multi-copy integrants, while that with the A. oryzae niaD-carrying plasmid occurred mainly by single-copy and homologous integration events (more than 50% frequency), indicating that with this transformation system, the transformation marker could be selected according to the integration pattern one desires.

Original languageEnglish
Pages (from-to)1367-1369
Number of pages3
JournalBioscience, Biotechnology and Biochemistry
Volume61
Issue number8
DOIs
Publication statusPublished - 1997 Jan 1
Externally publishedYes

Keywords

  • ATP sulfurylase
  • Aspergillus oryzae
  • Nitrate reductase
  • Transformation

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biotechnology
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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