Transformation of the industrial strain of Aspergillus oryzae with the homologous amdS gene as a dominant selectable marker

Katsuya Gomi, Katsuhiko Kitamoto, Chieko Kumagai

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

A homologous transformation system has been developed for Aspergillus oryzae industrial strains using the A. oryzae acetamidase-encoding gene (amdS) as a dominant selectable marker. Although the transformation efficiency is relatively low (0.4-1 transformants per μg DNA), the transformants contained significantly high copy numbers of the plasmid integrated into the genome. In addition, the promoter of the amdS gene was replaced by the strong expression promoter of the α-amylase-encoding gene (amyB) from A. oryzae, thereby enhancing the selection of the transformants.

Original languageEnglish
Pages (from-to)389-391
Number of pages3
JournalJournal of Fermentation and Bioengineering
Volume74
Issue number6
DOIs
Publication statusPublished - 1992 Jan 1
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

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