Transformation of radish (Raphanus sativus L.) via sonication and vacuum infiltration of germinated seeds with Agrobacterium harboring a group 3 LEA gene from B. napus

Byong Jin Park, Zaochang Liu, Akira Kanno, Toshiaki Kameya

Research output: Contribution to journalArticlepeer-review

43 Citations (Scopus)

Abstract

A protocol for producing transgenic radish (Raphanus sativus) was obtained by using both ultrasonic and vacuum infiltration assisted, Agrobacterium- mediated transformation. The Agrobacterium strain LBA4404 contained the binary vector pBI121-LEA (late embyogenesis abundant), which carried a Group 3 LEA gene, from Brassica napus. Among six combinations, Agrobacterium-mediated transformation assisted by a combination of 5-min sonication with 5-min vacuum infiltration resulted in the highest transformation frequency. The existence, integration and expression of transferred LEA gene in transgenic T1 plants were confirmed by PCR, genomic Southern and Western blot analysis. Transgenic radish demonstrated better growth performance than non-transformed control plants under osmotic and salt stress conditions. Accumulation of Group 3 LEA protein in the vegetative tissue of transgenic radish conferred increased tolerance to water deficit and salt stress.

Original languageEnglish
Pages (from-to)494-500
Number of pages7
JournalPlant Cell Reports
Volume24
Issue number8
DOIs
Publication statusPublished - 2005 Oct 1

Keywords

  • Agrobacterium-mediated transformation
  • Late embyogenesis abundant (LEA) protein
  • Radish (Raphanus sativus L.)
  • Sonication
  • Vacuum infiltration

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

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