Abstract
Mammalian whole embryo culture (WEC) was developed long before transgenic and gene targeted animals are widely used. Electroporation (EP) into cultured rodent embryos has expanded the potential to analyze gene functions in mammalian embryos by transferring exogenous plasmid vectors or small nucleotides in region- and stage-specific ways. This method is quite simple, and therefore enables us to analyze gene functions more quickly than genetic manipulation. In this review, we introduce combinatorial methods of WEC and EP, and summarize various applications in developmental neurobiology.
Original language | English |
---|---|
Pages (from-to) | 485-497 |
Number of pages | 13 |
Journal | Development Growth and Differentiation |
Volume | 50 |
Issue number | 6 |
DOIs | |
Publication status | Published - 2008 Aug |
Keywords
- Brain development
- Electroporation
- Mouse
- Rat
- Whole embryo culture
ASJC Scopus subject areas
- Developmental Biology
- Cell Biology