Transferring genes into cultured mammalian embryos by electroporation

Masanori Takahashi; Tadashi Nomura; Noriko Osumi

doi:10.1111/j.1440-169X.2008.01046.x

Transferring genes into cultured mammalian embryos by electroporation

Masanori Takahashi, Tadashi Nomura, Noriko Osumi

MED - United Centers for Advanced Research and Translational Medicine (ART)

Research output: Contribution to journal › Review article

29 Citations (Scopus)

Abstract

Mammalian whole embryo culture (WEC) was developed long before transgenic and gene targeted animals are widely used. Electroporation (EP) into cultured rodent embryos has expanded the potential to analyze gene functions in mammalian embryos by transferring exogenous plasmid vectors or small nucleotides in region- and stage-specific ways. This method is quite simple, and therefore enables us to analyze gene functions more quickly than genetic manipulation. In this review, we introduce combinatorial methods of WEC and EP, and summarize various applications in developmental neurobiology.

Original language	English
Pages (from-to)	485-497
Number of pages	13
Journal	Development Growth and Differentiation
Volume	50
Issue number	6
DOIs	https://doi.org/10.1111/j.1440-169X.2008.01046.x
Publication status	Published - 2008 Aug

Keywords

Brain development
Electroporation
Mouse
Rat
Whole embryo culture

ASJC Scopus subject areas

Developmental Biology
Cell Biology

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AU - Osumi, Noriko

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AB - Mammalian whole embryo culture (WEC) was developed long before transgenic and gene targeted animals are widely used. Electroporation (EP) into cultured rodent embryos has expanded the potential to analyze gene functions in mammalian embryos by transferring exogenous plasmid vectors or small nucleotides in region- and stage-specific ways. This method is quite simple, and therefore enables us to analyze gene functions more quickly than genetic manipulation. In this review, we introduce combinatorial methods of WEC and EP, and summarize various applications in developmental neurobiology.

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KW - Rat

KW - Whole embryo culture

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