Transfer of cholesterol and sitosterol from rat intestinal brush border membranes to phospholipid liposomes: Effects of SCP2

Ikuo Ikeda; Michihiro Sugano; Terence J. Scallen; George V. Vahouny; Linda L. Gallo

doi:10.1080/00021369.1990.10870358

Transfer of cholesterol and sitosterol from rat intestinal brush border membranes to phospholipid liposomes: Effects of SCP₂

Ikuo Ikeda, Michihiro Sugano, Terence J. Scallen, George V. Vahouny, Linda L. Gallo

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

Regarding the transport of absorbed sterols to endoplasmic reticulum in intestinal mucosal cells, transfer of cholesterol and sitosterol from rat intestinal brush border membranes to phospholipid liposomes was investigated. Transfer of sitosterol from brush border membranes to liposomes was significantly slower than that of cholesterol. Sitosterol transfer increased dose-dependently with the addition of sterol carrier protein₂ (SCP₂), but only a transient increase was observed with cholesterol. The results suggest that a slower rate of transfer of sitosterol than cholesterol from brush border membranes to the intracellular site of use may be one mechanism by which sitosterol transport into lymph is delayed, as observed previously in the rat intestine. SCP₂ may not essentially participate in the transfer of absorbed cholesterol.

Original language	English
Pages (from-to)	2649-2653
Number of pages	5
Journal	Agricultural and Biological Chemistry
Volume	54
Issue number	10
DOIs	https://doi.org/10.1080/00021369.1990.10870358
Publication status	Published - 1990 Oct
Externally published	Yes

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)
Agricultural and Biological Sciences(all)

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10.1080/00021369.1990.10870358

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title = "Transfer of cholesterol and sitosterol from rat intestinal brush border membranes to phospholipid liposomes: Effects of SCP2",

abstract = "Regarding the transport of absorbed sterols to endoplasmic reticulum in intestinal mucosal cells, transfer of cholesterol and sitosterol from rat intestinal brush border membranes to phospholipid liposomes was investigated. Transfer of sitosterol from brush border membranes to liposomes was significantly slower than that of cholesterol. Sitosterol transfer increased dose-dependently with the addition of sterol carrier protein2 (SCP2), but only a transient increase was observed with cholesterol. The results suggest that a slower rate of transfer of sitosterol than cholesterol from brush border membranes to the intracellular site of use may be one mechanism by which sitosterol transport into lymph is delayed, as observed previously in the rat intestine. SCP2 may not essentially participate in the transfer of absorbed cholesterol.",

author = "Ikuo Ikeda and Michihiro Sugano and Scallen, {Terence J.} and Vahouny, {George V.} and Gallo, {Linda L.}",

note = "Funding Information: Acknowledgment. Supported in part by USPHS grants: AM32309, HL 32982, and NIH SOI-RR-5359.",

year = "1990",

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number = "10",

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T1 - Transfer of cholesterol and sitosterol from rat intestinal brush border membranes to phospholipid liposomes

T2 - Effects of SCP2

AU - Ikeda, Ikuo

AU - Sugano, Michihiro

AU - Scallen, Terence J.

AU - Vahouny, George V.

AU - Gallo, Linda L.

N1 - Funding Information: Acknowledgment. Supported in part by USPHS grants: AM32309, HL 32982, and NIH SOI-RR-5359.

PY - 1990/10

Y1 - 1990/10

N2 - Regarding the transport of absorbed sterols to endoplasmic reticulum in intestinal mucosal cells, transfer of cholesterol and sitosterol from rat intestinal brush border membranes to phospholipid liposomes was investigated. Transfer of sitosterol from brush border membranes to liposomes was significantly slower than that of cholesterol. Sitosterol transfer increased dose-dependently with the addition of sterol carrier protein2 (SCP2), but only a transient increase was observed with cholesterol. The results suggest that a slower rate of transfer of sitosterol than cholesterol from brush border membranes to the intracellular site of use may be one mechanism by which sitosterol transport into lymph is delayed, as observed previously in the rat intestine. SCP2 may not essentially participate in the transfer of absorbed cholesterol.

AB - Regarding the transport of absorbed sterols to endoplasmic reticulum in intestinal mucosal cells, transfer of cholesterol and sitosterol from rat intestinal brush border membranes to phospholipid liposomes was investigated. Transfer of sitosterol from brush border membranes to liposomes was significantly slower than that of cholesterol. Sitosterol transfer increased dose-dependently with the addition of sterol carrier protein2 (SCP2), but only a transient increase was observed with cholesterol. The results suggest that a slower rate of transfer of sitosterol than cholesterol from brush border membranes to the intracellular site of use may be one mechanism by which sitosterol transport into lymph is delayed, as observed previously in the rat intestine. SCP2 may not essentially participate in the transfer of absorbed cholesterol.

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JF - Bioscience, Biotechnology and Biochemistry

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Transfer of cholesterol and sitosterol from rat intestinal brush border membranes to phospholipid liposomes: Effects of SCP₂

Abstract

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