Transcriptionally active heterodimer formation of an Arnt-like PAS protein, Arnt3, with HIF-1a, HLF, and clock

Sho Takahata, Kazuhiro Sogawa, Akira Kobayashi, Masatsugu Ema, Junsei Mimura, Nobuhiro Ozaki, Yoshiaki Fujii-Kuriyama

    Research output: Contribution to journalArticle

    105 Citations (Scopus)

    Abstract

    We isolated a cDNA clone encoding a polypeptide of 626 amino acids containing basic helix-loop-helix (bHLH) and PAS domains from a mouse cDNA library of P19 cells. This protein, termed Arnt3, showed the highest similarity to Arnt and Arnt2 in the bHLH and PAS regions. Arnt3 mRNA was expressed in brain, skeletal muscle, 13.5 day embryos, and P19 cells treated with retinoic acid.The partner PAS proteins of Arnt3 were searched for by the two-hybrid system in yeast, and HIF-1α, HLF, and Clock among various bHLH/PAS proteins were found. Gel mobility shift analysis using nuclear extracts from 293T cells cotransfected with Arnt3 and HIF-1α (or HLF) expression plasmids revealed that these complexes specifically bound the hypoxia-response element (HRE). Coexpression of Arnt3 and HIF-1α (or HLF) in Arnt-deficient c4 cells enhanced transcription of a reporter gene driven by the HRE sequences, We also showed that Arnt3 contained an activation domain at the C-terminal region and a repression domain between the PAS-A and PAS-B regions.

    Original languageEnglish
    Pages (from-to)789-794
    Number of pages6
    JournalBiochemical and biophysical research communications
    Volume248
    Issue number3
    DOIs
    Publication statusPublished - 1998 Jul 30

    ASJC Scopus subject areas

    • Biophysics
    • Biochemistry
    • Molecular Biology
    • Cell Biology

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