Transcriptional activity of nuclei from WI‐38 cells at various passages

Kiyoshi Nose, Hiroshi Okamoto

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

Transcriptional activity of nuclei from human diploid fibroblast (WI‐38) cells at different passage levels was studied with endogenous RNA polymerase. The rate and extent of the RNA synthesis decreased when the nuclei were were prepared from senescent cells. The decreased activity of RNA synthesis in senescent cell nuclei was demonstrated from both the transcriptional assay and the estimation of the processing activity, i.e., polyadenylation of the transcripts.

Original languageEnglish
Pages (from-to)51-54
Number of pages4
JournalJournal of Cellular Physiology
Volume102
Issue number1
DOIs
Publication statusPublished - 1980 Jan
Externally publishedYes

ASJC Scopus subject areas

  • Physiology
  • Clinical Biochemistry
  • Cell Biology

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