TY - JOUR
T1 - Transcription-independent role of Bach1 in mitosis through a nuclear exporter Crm1-dependent mechanism
AU - Li, Jie
AU - Shiraki, Takuma
AU - Igarashi, Kazuhiko
N1 - Funding Information:
We would like to thank Drs. Andrey Brydun, Etsuro Itoh, Kozo Tanaka and Tsuyoshi Ikura for providing us with experimental materials. This study was supported by Grants-in-Aid and the Network Medicine Global COE Program from the Ministry of Education, Culture, Sport, Science and Technology of Japan. The authors declare that they have no conflict of interest.
Copyright:
Copyright 2013 Elsevier B.V., All rights reserved.
PY - 2012/2/17
Y1 - 2012/2/17
N2 - The transcriptional repressor Bach1 mediates various stress responses. Despite its role in transcription, Bach1 is predominantly exported to the cytoplasm in a Crm1-dependent manner, but the functional role of its cytoplasmic retention is still unclear. We found that Bach1 was also excluded from mitotic chromatin by a C-terminal cytoplasmic localization sequence dependent and leptomycin B sensitive process. Bach1 depletion resulted in disordered mitotic chromosome alignment, which was rescued by Bach1 mutants lacking the BTB or DNA binding domains, suggesting its transcription-independent mechanism. We thus revealed a novel role of Bach1 in the regulation of mitotic chromosome dynamics.
AB - The transcriptional repressor Bach1 mediates various stress responses. Despite its role in transcription, Bach1 is predominantly exported to the cytoplasm in a Crm1-dependent manner, but the functional role of its cytoplasmic retention is still unclear. We found that Bach1 was also excluded from mitotic chromatin by a C-terminal cytoplasmic localization sequence dependent and leptomycin B sensitive process. Bach1 depletion resulted in disordered mitotic chromosome alignment, which was rescued by Bach1 mutants lacking the BTB or DNA binding domains, suggesting its transcription-independent mechanism. We thus revealed a novel role of Bach1 in the regulation of mitotic chromosome dynamics.
KW - Chromosome alignment
KW - Leptomycin B
KW - Nucleocytoplasmic transport
KW - Oxidative stress
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U2 - 10.1016/j.febslet.2012.01.028
DO - 10.1016/j.febslet.2012.01.028
M3 - Article
C2 - 22289179
AN - SCOPUS:84862810861
VL - 586
SP - 448
EP - 454
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 4
ER -