Transcription-independent role of Bach1 in mitosis through a nuclear exporter Crm1-dependent mechanism

Jie Li, Takuma Shiraki, Kazuhiko Igarashi

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

The transcriptional repressor Bach1 mediates various stress responses. Despite its role in transcription, Bach1 is predominantly exported to the cytoplasm in a Crm1-dependent manner, but the functional role of its cytoplasmic retention is still unclear. We found that Bach1 was also excluded from mitotic chromatin by a C-terminal cytoplasmic localization sequence dependent and leptomycin B sensitive process. Bach1 depletion resulted in disordered mitotic chromosome alignment, which was rescued by Bach1 mutants lacking the BTB or DNA binding domains, suggesting its transcription-independent mechanism. We thus revealed a novel role of Bach1 in the regulation of mitotic chromosome dynamics.

Original languageEnglish
Pages (from-to)448-454
Number of pages7
JournalFEBS Letters
Volume586
Issue number4
DOIs
Publication statusPublished - 2012 Feb 17

Keywords

  • Chromosome alignment
  • Leptomycin B
  • Nucleocytoplasmic transport
  • Oxidative stress

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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