Transcription factor Tbx6 plays a central role in fate determination between mesenchyme and muscle in embryos of the ascidian, Halocynthia roretzi

Gaku Kumano, Nobue Negoro, Hiroki Nishida

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

Developmental fates are determined in relation to other fates in surrounding cells within the embryo, creating diversified tissue or cell types in the course of development. In ascidian embryos, maternally localized factors and inductive signals play essential and cooperative roles in fate determination at appropriate spatial and temporal positions during embryogenesis. Here, to clarify fate determination mechanisms of mesenchyme and muscle during mesenchyme induction by the fibroblast growth factor (FGF) signal in Halocynthia roretzi, we examined the function of transcription factor Tbx6, which acts as a pivotal mediator of the maternally localized muscle differentiation determinant Macho-1. Our results suggest that the level of Tbx6 expression increases in muscle lineage cells through positive feedback regulation that promotes muscle differentiation as well as mesenchymal fate suppression. In addition, the FGF signal inactivated Tbx6 transcriptional activity and positive feedback, leading to induction of the mesenchymal lineage. Taken together, our finding suggests that Tbx6 is an important factor for determining mesenchyme and muscle fates.

Original languageEnglish
Pages (from-to)310-322
Number of pages13
JournalDevelopment Growth and Differentiation
Volume56
Issue number4
DOIs
Publication statusPublished - 2014 May

Keywords

  • Ascidian
  • Induction
  • Mesenchyme
  • Muscle
  • Tbx6

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

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