Transcription factor NFE2L2/NRF2 is a regulator of macroautophagy genes

Marta Pajares, Natalia Jiménez-Moreno, Ángel J. García-Yagüe, Maribel Escoll, María L. de Ceballos, Fred Van Leuven, Alberto Rábano, Masayuki Yamamoto, Ana I. Rojo, Antonio Cuadrado

Research output: Contribution to journalArticle

110 Citations (Scopus)

Abstract

Autophagy is a highly coordinated process that is controlled at several levels including transcriptional regulation. Here, we identify the transcription factor NFE2L2/NRF2 (nuclear factor, erythroid 2 like 2) as a regulator of autophagy gene expression and its relevance in a mouse model of Alzheimer disease (AD) that reproduces impaired APP (amyloid β precursor protein) and human (Hs)MAPT/TAU processing, clearance and aggregation. We screened the chromatin immunoprecipitation database ENCODE for 2 proteins, MAFK and BACH1, that bind the NFE2L2-regulated enhancer antioxidant response element (ARE). Using a script generated from the JASPAR's consensus ARE sequence, we identified 27 putative AREs in 16 autophagy-related genes. Twelve of these sequences were validated as NFE2L2 regulated AREs in 9 autophagy genes by additional ChIP assays and quantitative RT-PCR on human and mouse cells after NFE2L2 activation with sulforaphane. Mouse embryo fibroblasts of nfe2l2-knockout mice exhibited reduced expression of autophagy genes, which was rescued by an NFE2L2 expressing lentivirus, and impaired autophagy flux when exposed to hydrogen peroxide. NFE2L2-deficient mice co-expressing HsAPPV717I and HsMAPTP301L, exhibited more intracellular aggregates of these proteins and reduced neuronal levels of SQSTM1/p62, CALCOCO2/NDP52, ULK1, ATG5 and GABARAPL1. Also, colocalization of HsAPPV717I and HsMAPTP301L with the NFE2L2-regulated autophagy marker SQSTM1/p62 was reduced in the absence of NFE2L2. In AD patients, neurons expressing high levels of APP or MAPT also expressed SQSTM1/p62 and nuclear NFE2L2, suggesting their attempt to degrade intraneuronal aggregates through autophagy. This study shows that NFE2L2 modulates autophagy gene expression and suggests a new strategy to combat proteinopathies.

Original languageEnglish
Pages (from-to)1902-1916
Number of pages15
JournalAutophagy
Volume12
Issue number10
DOIs
Publication statusPublished - 2016 Oct 2

Keywords

  • Alzheimer disease
  • Tau
  • amyloid precursor protein
  • neurodegenerative diseases
  • neuroprotection
  • oxidative stress
  • proteostasis

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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  • Cite this

    Pajares, M., Jiménez-Moreno, N., García-Yagüe, Á. J., Escoll, M., de Ceballos, M. L., Van Leuven, F., Rábano, A., Yamamoto, M., Rojo, A. I., & Cuadrado, A. (2016). Transcription factor NFE2L2/NRF2 is a regulator of macroautophagy genes. Autophagy, 12(10), 1902-1916. https://doi.org/10.1080/15548627.2016.1208889