TY - JOUR
T1 - Transcranial optogenetic stimulation for functional mapping of the motor cortex
AU - Hira, Riichiro
AU - Honkura, Naoki
AU - Noguchi, Jun
AU - Maruyama, Yoshio
AU - Augustine, George J.
AU - Kasai, Haruo
AU - Matsuzaki, Masanori
N1 - Funding Information:
We thank Y. Iwanami, R. Takizawa, C. Miura, and R. Matsuura for technical assistance, and K. Kitamura, Y. Matsuzaka, and T. Nakajima for helpful discussions and advice. This work was partly supported by Grants-in-Aid from the Ministry of Education, Culture, Sports, Science, and Technology (MEXT) of Japan (M.M. and H.K.), the Global COE Program (Integrative life Science) of MEXT (H.K), the National Institutes of Health Grants (H.K. and G.J.A.), and the Human Frontier Science Program (RPG0071/2002 to H.K.).
PY - 2009/5/15
Y1 - 2009/5/15
N2 - We developed a method that uses Channelrhodopsin-2 (ChR2) for transcranial optogenetic stimulation. This method is based on scanning a light beam over the brain, thereby photostimulating ChR2-expressing neurons in intact mice. As a proof of principle, we applied this technique to the motor cortex of transgenic mice expressing ChR2 in cortical pyramidal cells. Photostimulation induced limb movements that were time-locked with millisecond precision and could be induced at frequencies up to 20 Hz. By scanning this light beam, we could map the distribution of neurons associated with limb movement. With this approach we could simultaneously define motor maps controlling two limbs and could reproducibly generate such cortical motor maps over periods of weeks. This method allows non-invasive mapping of brain circuitry in living animals and could help define the connection between behavior and brain circuitry.
AB - We developed a method that uses Channelrhodopsin-2 (ChR2) for transcranial optogenetic stimulation. This method is based on scanning a light beam over the brain, thereby photostimulating ChR2-expressing neurons in intact mice. As a proof of principle, we applied this technique to the motor cortex of transgenic mice expressing ChR2 in cortical pyramidal cells. Photostimulation induced limb movements that were time-locked with millisecond precision and could be induced at frequencies up to 20 Hz. By scanning this light beam, we could map the distribution of neurons associated with limb movement. With this approach we could simultaneously define motor maps controlling two limbs and could reproducibly generate such cortical motor maps over periods of weeks. This method allows non-invasive mapping of brain circuitry in living animals and could help define the connection between behavior and brain circuitry.
KW - Channelrhodopsin-2
KW - Mapping
KW - Motor cortex
KW - Photostimulation
UR - http://www.scopus.com/inward/record.url?scp=63249097867&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=63249097867&partnerID=8YFLogxK
U2 - 10.1016/j.jneumeth.2009.02.001
DO - 10.1016/j.jneumeth.2009.02.001
M3 - Article
C2 - 19428535
AN - SCOPUS:63249097867
VL - 179
SP - 258
EP - 263
JO - Journal of Neuroscience Methods
JF - Journal of Neuroscience Methods
SN - 0165-0270
IS - 2
ER -