Transcellular transport of organic anions across a double-transfected Madin-Darby canine kidney II cell monolayer expressing both human organic anion-transporting polypeptide (OATP2/SLC21A6) and multidrug resistance-associated protein 2 (MRP2/ABCC2)

Makoto Sasaki, Hiroshi Suzuki, Kousei Ito, Takaaki Abe, Yuichi Sugiyama

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Abstract

Human organic anion transporting polypeptide 2 (OATP2/SLC21A6) and multidrug resistance-associated protein 2 (MRP2/ABCC2) play important roles in the vectorial transport of organic anions across hepatocytes. In the present study, we have established a double-transfected Madin-Darby canine kidney (MDCK II) cell monolayer, which expresses both OATP2 and MRP2 on basal and apical membranes, respectively. The basal-to-apical transport of 17β estradiol 17β-D-glucuronide (E 217βG), pravastatin, and leukotriene C 4 (LTC 4), which are substrates of OATP2 and MRP2, was significantly higher than that in the opposite direction in the double-transfected cells. Such vectorial transport was also observed for taurolithocholate sulfate, which is transported by rat oatpl and Mrp2. The K m values of E 217βG and pravastatin for the basal-to-apical flux were 27.9 and 24.3 μM, respectively, which were comparable with those reported for OATP2. Moreover, the MRP2-mediated export of E 217βG across the apical membrane was not saturated. In contrast, basal-to-apical transport of estrone-3-sulfate and dehydroepiandrosterone sulfate, which are significantly transported by OATP2, but not by MRP2, was not stimulated by MRP2 expression. The double-transfected MDCK II monolayer expressing both OATP2 and MRP2 may be used to analyze the hepatic vectorial transport of organic anions and to screen the transport profiles of new drug candidates.

Original languageEnglish
Pages (from-to)6497-6503
Number of pages7
JournalJournal of Biological Chemistry
Volume277
Issue number8
DOIs
Publication statusPublished - 2002 Feb 22

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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