Trafficking to the Outer Polar Domain Defines the Root-Soil Interface

Łukasz Łangowski, Kamil Růžička, Satoshi Naramoto, Jürgen Kleine-Vehn, Jiří Friml

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

In animals, the interface between organism and environment is constituted by the epithelium [1]. In plants, the exchange of nutrients and signals between root and soil is crucial for their survival, but the cellular mechanisms underlying the epithelium-like function and specific localization of proteins to the root surface have not been identified [2]. Here we analyze the mechanism of polar delivery to the root-soil interface of the proteins BOR4, ABCG37, and PEN3, which transport nutrients [2], transport plant hormones, and are required for pathogen defense [3], respectively. The simultaneous visualization of these proteins and the apical and basal cargos in a single cell demonstrates that the outermost cell side represents an additional polar domain. Delivery to this outer polar domain depends on ARF GEF [4] and actin [5-8] function but does not require known molecular components of the apical or basal targeting. The outer polar delivery is, in contrast to known basal and apical cargos [9, 10], mediated by the polar secretion. Our findings show that the outermost cell membranes of roots define an additional polar domain in plant cells along with a specific, previously uncharacterized, polar targeting mechanism that is important for defining the functional, epithelium-like root-soil interface.

Original languageEnglish
Pages (from-to)904-908
Number of pages5
JournalCurrent Biology
Volume20
Issue number10
DOIs
Publication statusPublished - 2010 May 25
Externally publishedYes

Keywords

  • CELLBIO

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

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    Łangowski, Ł., Růžička, K., Naramoto, S., Kleine-Vehn, J., & Friml, J. (2010). Trafficking to the Outer Polar Domain Defines the Root-Soil Interface. Current Biology, 20(10), 904-908. https://doi.org/10.1016/j.cub.2010.03.059